在大动脉剖析中与PANoptosis相关的基因生物标志物
Yuting Pu1, Yang Zhou2, Tuo Guo1
1Department of Emergency Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan, 410011, China; Emergency Medicine and Difficult Disease Institute, The Second Xiangya Hospital, Central South University, Changsha, Hunan, 410011, China.
Archives of biochemistry and biophysics
|March 14, 2025
概括
血管光滑肌细胞 (VSMC) 的编程细胞死亡在大动脉剖析 (AD) 中至关重要. 这项研究发现VSMCs中增加了PANoptosis (编程细胞死亡),突出了GADD45B,CDKN1A和SOD2作为AD病变发生的关键参与者.
科学领域:
- 心血管生物学 心血管生物学
- 细胞死亡机制 细胞死亡机制
- 基因组学就是基因组学.
背景情况:
- 血管光滑肌细胞 (VSMCs) 的编程细胞死亡在大动脉剖析 (AD) 病变发生过程中至关重要.
- 在阿尔茨海默病中,PANoptosis的特定作用,包括pyroptosis,apoptosis和necroptosis,仍然在很大程度上未被定义.
研究的目的:
- 在AD期间调查VSMC中PANoptosis的水平和意义.
- 确定参与PANoptosis的关键基因,这些基因有助于AD发展.
主要方法:
- 对单细胞测序数据集 (GSE213740,GSE153434,GSE147026,GSE52093) 的分析,以评估 PANoptosis 评分和识别差异表达基因 (DEG).
- 权重基因共同表达网络分析,基因本体学,KEGG通路分析和蛋白质-蛋白质相互作用分析.
- 在人类AD组织和小鼠模型中验证关键基因.
主要成果:
- 从AD患者的VSMC中观察到高的PANoptosis得分.
- 确定了19种与PANoptosis相关的DEGs (PANDEGs),影响VSMC分化,DNA损伤反应和亡.
- 关键的PANDEGs,包括GADD45B,CDKN1A和SOD2,得到了验证,并与α-SMA一起表达,与免疫细胞透相关,并突出了P53和TGF-β通路.
结论:
- 在VSMC中增加的PANoptosis与AD的发病因子有关.
- GADD45B,CDKN1A和SOD2被确定为通过参与PANoptosis的关键基因,有助于AD发展.
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