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通过应用昆虫肥料来缓解抗生素耐药性基因的垂直迁移和泄漏风险

Zhengzheng Zhao¹, Bingqi Gao¹, Gen Li¹

¹National Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, National Engineering Research Center of Microbial Pesticides, Huazhong Agricultural University, Wuhan, 430070, PR China; Hubei Hongshan Laboratory, Wuhan, 430070, Hubei, PR China.

Environmental research

|March 14, 2025

概括

黑兵有机肥 (BOF) 显著减少抗生素耐药性基因 (ARG) 入地下水. BOF促进有益的细菌,减轻ARG,并提供可持续的受精替代品.

科学领域:

环境科学环境科学
微生物学微生物学
农业科学农业科学

背景情况:

土壤中的抗生素耐药性基因 (ARG) 对生态和公共健康构成风险.
传统的肥料可能会导致ARG出.
来自昆虫的有机肥是一种可持续的替代品,具有潜在较低的ARG风险.

研究的目的:

评估黑士兵有机肥 (BOF) 对ARG出的影响.
为了比较BOF和控制有机肥 (COF) 之间的ARG出.
确定缓解ARG迁移的机制和指标.

主要方法:

进行了土壤柱漏实验.
在液中量化了ARG度.
随机森林,网络和地幔测试被用于分析.
线性回归确定了与ARG减少相关的关键基因.

主要成果:

与COF相比,BOF减少了ARG水的22.1%-49.3%,而COF则减少了22.1%-49.3%.
BOF促进了有益的细菌物种的浸出.
细菌生物膜的形成与ARG缓解有关.
特定的Bacillus生物膜基因 (pgaD, slrR, kpsC) 被确定为关键指标.

结论:

BOF有效地降低了ARG漏和垂直迁移风险.
增强的代谢和Bacillus生物膜的形成是关键机制.
细菌生物膜相关的基因作为地下水ARG监测的可靠指标.

关键词:

抗生素耐药性基因是抗生素耐药性的基因.细菌杆菌是一种昆虫肥料是一种昆虫肥料.泄漏的风险泄漏的风险垂直迁移是指垂直迁移的过程.

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