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Updated: May 22, 2025

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普特雷辛的基因毒性及其对HepG2细胞系基因表达的影响

Franco Dani Campos-Pereira¹, Letícia Rocha Gonçalves¹, Raquel Vaz Hara Jardim¹

¹Universidade Estadual Paulista "Júlio de Mesquita Filho" - Campus Rio Claro, Instituto de Biociências, Departamento de Biologia Geral e Aplicada, Avenida 24-A, n° 1515, Bela Vista, CEP 13506-900 Rio Claro, São Paulo, Brazil.

Toxicology in vitro : an international journal published in association with BIBRA

|March 14, 2025

概括

腐烂的尸体中的死体液含有有毒的生物原氨酸,如. 这项研究发现,布特雷辛会导致人类细胞中显著的DNA损伤和遗传改变,造成墓地污染物的环境风险.

科学领域:

环境毒理学环境毒理学
分子毒理学分子毒理学
生物化学生物化学

背景情况:

腐烂的人类遗骸产生死体液,一种含有有害生物氨基酸的有毒液体.
普特瑞辛是一种常见的生物氨基,主要研究其在食品中的作用,而不是作为环境污染物.
了解死体排泄物成分的环境风险对于公共卫生和生态安全至关重要.

研究的目的:

为了研究不同度的生物氨基普特雷辛对人类HepG2细胞的基因毒性作用.
评估普特雷斯作为源自死体泄露的环境污染物的潜力.
提供作为环境污染物的雷素毒性的第一次评估.

主要方法:

暴露HepG2细胞不同度的普特雷辛 (0.53.2毫米).
利用彗星测试来测量DNA损伤 (尾巴DNA强度).
使用微核测试来检测染色体损伤 (微核,核芽,染色体桥梁).
分析了与氧化应激和DNA损伤途径相关的基因表达改变.

主要成果:

在彗星测试中,所有测试的度都观察到显著的DNA损伤.
微核测试显示微核,核芽和染色体桥梁的显著增加.
检测到与氧化应激和DNA损伤相关的基因表达的统计学上显著的变化.

结论:

普特雷辛在人类的HepG2细胞中表现出基因毒性潜力,这表明存在死体泄漏的风险.
这项研究强调了与墓地污染物相关的职业和环境风险.
进一步的研究是必要的,以了解墓地污染对环境健康的全部影响.

关键词:

彗星测量试验方法修复DNA损伤的修复微核试验微核试验尼克罗 - 泄漏器氧化应激是一种氧化应激.脏是一种腐蚀性.

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