聚烯胺对挥发性脂肪酸积累的新见解:泥粉碎机制和微生物社区功能功能
Guorun Zhou1, Xiangji Kong2, Daojing Zeng1
1Collaborative Innovation Center of Atmospheric Environment and Equipment Technology, Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control, School of Environmental Science and Engineering, Nanjing University of Information Science & Technology, Nanjing 210044, China.
Bioresource technology
|March 14, 2025
概括
在废弃活性污泥 (WAS) 发酵中添加的多烯胺 (PAM) 抑制了细胞破坏和有机物分解. 这导致挥发性脂肪酸 (VFA) 生产减少,但有利于酸的形成.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 生物化学 生化学
背景情况:
- 废弃活性污泥 (WAS) 的无氧发酵对于资源回收至关重要.
- 了解聚烯胺 (PAM) 等添加剂的影响对于优化工艺至关重要.
研究的目的:
- 调查聚烯胺 (PAM) 在废物活性污泥 (WAS) 无氧发酵过程中对水解,酸化和微生物群体动态的影响.
- 阐明PAM影响挥发性脂肪酸 (VFA) 生产的机制.
主要方法:
- 用四种不同度的多烯胺 (PAM) 补充WAS发酵系统.
- 分析水解,酸化,挥发性脂肪酸 (VFA) 生产,酶活动 (蛋白酶,α-葡萄糖酶) 和微生物群落结构 (丰富性,多样性,类的相对丰富性).
主要成果:
- 聚烯胺 (PAM) 抑制了废物活性污泥 (WAS) 细胞破坏,减少了总体挥发性脂肪酸 (VFA) 产量.
- PAM增加了VFA中酸的比例,降低了蛋白酶和α-葡萄糖酶活动,并抑制了有机基质分解.
- 在微生物学上,PAM减少了微生物的丰富性和多样性,但丰富了Bacteroidota和Actinobacteriota,促进了酸的产生.
结论:
- 聚烯胺 (PAM) 通过抑制细胞破坏和关键酶活动,对废弃活性污泥 (WAS) 发酵中的水解和酸化产生负面影响.
- PAM改变了微生物社区结构,有利于增加酸积累的特定类型.
- 这些发现提供了对PAM在无氧污泥发酵过程中挥发性脂肪酸 (VFA) 生产中的作用的理论见解.
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