相关实验视频

Updated: May 22, 2025

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Published on: July 6, 2021

可编程的固态冷凝剂用于对哺乳动物基因表达的时空控制

Yukai Wang^1,2,3,4, Jian Jiang^1,3,5,6, Qiqi Xiong^1,3,5,6

¹School of Life Sciences, Fudan University, Shanghai, China.

Nature chemical biology

|March 15, 2025

概括

研究人员开发了一种新的方法来控制基因表达,使用化学诱导核凝聚物. 这一策略允许精确的,按需调节哺乳动物细胞中的转录和翻译.

科学领域:

分子生物学分子生物学
合成生物学合成生物学
生物化学生物化学

背景情况:

对哺乳动物基因表达的精确控制对于研究和治疗至关重要.
目前用于诱导基因表达的方法往往在特异性和时间上面临限制.
工程核冷凝剂为先进的基因调节提供了一个有希望的途径.

研究的目的:

开发一种用于化学编程的,依赖带的基因表达的多功能策略.
证明在各种细胞阶段和位置调节基因表达的能力.
通过在核凝聚物中保留mRNA来建立翻译控制的新方法.

主要方法:

利用生物分子凝结物的相分离特性.
工程合成基蛋白来将凝聚物结构连接到细胞区或基因产品.
使用合成固态冷凝剂进行诱导性基因调节.
将该策略应用于内源性和环节性基因位点.

主要成果:

使用工程基蛋白和合成凝聚剂,实现了转录和转化活动的诱导调节.
已证明与CRISPR-Cas9活动和转录沉默的最先进策略具有可比或优越的性能.
作为一种高效的转化调节方法,在核凝聚物中展示了化学诱导的mRNA保留.

结论:

开发的基于凝聚物的策略为精确,按需控制哺乳动物基因表达提供了多功能和有效的平台.
这种方法比现有方法具有显著的优势,特别是在实现转录和翻译调节方面.
化学可诱导的核凝聚物是促进合成生物学和基因治疗应用的强大工具.

更多相关视频

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

Stereotaxic Injection of a Viral Vector for Conditional Gene Manipulation in the Mouse Spinal Cord

Stereotaxic Injection of a Viral Vector for Conditional Gene Manipulation in the Mouse Spinal Cord

Published on: March 18, 2013

相关概念视频

Combinatorial Gene Control

Combinatorial Gene Control

Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...

Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...