在莫比亚,莫桑比克中部的Anopheles funestus中,多种杀虫剂耐药性
Caroline Kiuru1,2, Luis Constantino3, Gildo Cole3
1Centro de Investigação Em Saúde de Manhiça (CISM), Maputo, Mozambique. carolinekiuru@gmail.com.
Malaria journal
|March 15, 2025
概括
莫桑比克的疟疾蚊子表现出对DDT和 pyrethroids等关键杀虫剂的耐药性. 这一发现需要探索新的杀虫剂类别,以获得有效的载体控制计划.
科学领域:
- 医学昆虫学 医学昆虫学
- 分子昆虫学分子昆虫学
- 公共卫生昆虫学 昆虫学
背景情况:
- 疟疾载体的抗虫剂耐药性威胁到当前的控制方法,如用杀虫剂处理的网 (ITNs) 和室内残留喷雾 (IRS).
- 监测杀虫剂耐药性对于指导疟疾控制计划中的杀虫剂选择至关重要.
- 这项研究重点关注Anopheles funestus s.l.的抗虫剂耐药性概况. 在莫比亚地区,莫桑比克.
研究的目的:
- 为了确定Anopheles funestus s.l.的抗虫剂耐药性状态. 在莫比亚地区,莫桑比克.
- 研究这种关键疟疾载体中抗昆虫剂耐药性的潜在机制.
- 为该地区的传媒控制策略提供数据.
主要方法:
- 从莫佩亚地区的哨兵地点收集Anopheles成人和幼虫 (2021年10月-2022年9月).
- 使用世卫组织生物测试对bendiocarb,DDT,deltamethrin和pirimiphos-methyl进行杀虫剂敏感性测试.
- 与皮佩罗尼尔布托氧化物 (PBO) 和聚合酶链反应 (PCR) 进行协同生物测试,以评估耐药性机制和遗传突变 (A296S-Rdl,L119F-GSTe2,6.5 kb SV插入).
主要成果:
- 对bendiocarb (37%的死亡率),DDT (79%的死亡率),deltamethrin (14%的死亡率) 和pirimiphos-methyl (67%的死亡率) 观察到的表型耐药性.
- 皮佩罗尼尔丁氧化物 (PBO) 部分恢复了对deltamethrin的敏感性 (80%的死亡率),表明了代谢抵抗.
- 检测到抗性突变的高频率:A296S-Rdl为0.49,L119F-GSTe2为0.05,6.5 kb SV插入为0.92.
结论:
- 在Mopeia的Anopheles载体表现出对公共卫生中使用的四大杀虫剂类 (甲,有机酸盐,有机化物,碳酸盐) 的耐药性.
- 这些发现强调了在载体控制干预中迫切需要新型杀虫剂类别.
- 抵抗机制可能涉及代谢酶,如细胞P450和谷氨S转移酶,正如PBO协同作用和突变频率所表明的那样.
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