在粘膜皮质瘤癌中表达invadopodia相关蛋白质表达
Felippe José Almeida Loureiro1,2, Karolyny Martins Balbinot1, Maria Sueli da Silva Kataoka1
1Department of Oral Pathology, School of Dentistry, Federal University of Pará, Belém, Brazil.
Oral diseases
|March 15, 2025
概括
包括Tks4和Tks5在内的与invadopodia相关的蛋白质在粘膜皮质类癌症中表达很高,导致瘤的侵入性. 沉默这些蛋白质减少了癌细胞的入侵和 invadopodia 活动.
科学领域:
- 在瘤学瘤学.
- 细胞生物学 细胞生物学
- 癌症研究 癌症研究
背景情况:
- 粘膜腺癌 (MEC) 是最常见的唾液腺恶性瘤.
- 瘤的侵入性是癌症进展和转移的一个关键因素.
- 侵入者是富含活性蛋白的细胞突起,对于细胞外基质降解和入侵至关重要.
研究的目的:
- 为了研究MEC中与invadopodia相关的蛋白质的表达.
- 确定这些蛋白质在MEC侵入性中的作用.
- 探索针对这些蛋白质治疗策略的潜力.
主要方法:
- 免疫组织化学用于分析Tks4,Tks5,皮质素和MT1-MMP表达在27个MEC样本和10个正常的唾液腺对照中.
- 免疫光检测证实了MEC细胞系中的蛋白质表达.
- 在Tks4和Tks5基因沉默后进行了体外入侵和invadopodia形成试验.
主要成果:
- 在MEC组织中,与正常对照组相比,观察到与invadopodia相关的蛋白质的显著更高的表达.
- 在MEC细胞系中检测到Tks4和Tks5.
- 击败Tks4和Tks5显著降低了MEC细胞中的凝降解和侵入活动.
结论:
- 与invadopodia相关的蛋白质在MEC中受到上调,并有助于其侵入性表型.
- Tks4和Tks5是MEC入侵性的关键媒介,通过入侵.
- 准Tks4和Tks5可能是管理侵入性MEC的治疗方法.
相关概念视频
Cancer Cell Migration through Invadopodia
2.3K
Invadosome is a broad category of cell surface structures with proteolytic activity that degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
2.3K
Metastasis
5.5K
Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
5.5K
Abnormal Proliferation
4.4K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.4K
Cadherins in Tissue Organization
2.9K
The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...
Cell Sorting During Development
Cell sorting plays an...
2.9K
Mitogens and the Cell Cycle
6.4K
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
6.4K
Types of Membrane Protrusions
2.8K
The protrusion of the cell surface is an initial step for several cellular processes, including cell migration, phagocytosis, and neurite outgrowth. These membrane protrusions are a result of cytoskeletal rearrangement. The most widely observed cell protrusions include lamellipodia, pseudopodia, filopodia, microvilli, invadopodia, and podosomes. These protrusions can be of two types — static or dynamic.
The microvilli, an example of stable protrusions, are finger-like projections...
The microvilli, an example of stable protrusions, are finger-like projections...
2.8K


