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柏巴胺通过抑制脊柱TMEM34/SGK1/FOXO3轴来缓解神经病痛

Chang-Heng Yao¹, Zi-Meng Zhang¹, Shen Zhang¹

¹Jiangsu Key Laboratory of TCM Evaluation and Translational Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu 211198, China.

Phytomedicine : international journal of phytotherapy and phytopharmacology

|March 15, 2025

概括

这项研究确定了跨膜34 (TMEM34) 作为神经病痛 (NP) 的关键因素. 用贝巴 (BBM) 阻断TMEM34为NP提供了一个有前途的新疗法.

科学领域:

神经科学是一个神经科学.
药理学药理学是指药理学的学科.
分子生物学分子生物学

背景情况:

神经病变性疼痛 (NP) 的发病原因尚不清楚,有效和安全的治疗方法有限.
探索新的治疗点和天然化合物对于NP管理至关重要.

研究的目的:

确定神经病痛 (NP) 的新治疗点.
从传统中医药中发现针对已识别的途径的止痛药物.

主要方法:

在大鼠中建立了慢性收缩损伤 (CCI) 模型,用于行为分析.
使用了初级神经元,质细胞和细胞系 (PC12,C6,BV2) 的体外试验.
采用RNA测序 (RNA-Seq),免疫光,西斑和qRT-PCR来分析分子机制.

主要成果:

脊髓跨膜34 (TMEM34) 表达在CCI诱导的NP中增加;它的敲击减轻了NP,而过度表达加剧了它.
TMEM34激活脊髓神经元和星体细胞,与血清和葡萄糖皮质体诱导激酶-1 (SGK1) 相互作用,以抑制叉头盒O3 (FOXO3) 激活.
鉴定出来自Stephania epigaea H. S. Lo的柏巴胺 (BBM) 阻断了TMEM34/SGK1/FOXO3轴,有效地减少了NP.

结论:

TMEM34是神经病痛发展的关键因素.
柏巴胺 (BBM) 通过抑制TMEM34.4显示了作为NP治疗剂的潜力.

关键词:

星球细胞是星球细胞.中心敏感化中心这些神经元是神经元.神经病变性疼痛神经病变性疼痛在SGK1/FOXO3中使用.在TMEM34中使用TMEM34.

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