人类富血小板血通过PI3K/Akt信号通路促进原始卵泡的激活
Yashuang Weng1,2, Wenbo Zhang1, Fan Qu2
1The Innovation Centre of Ministry of Education for Development and Diseases, School of Medicine, South China University of Technology, Guangzhou, China.
Molecular human reproduction
|March 15, 2025
概括
人类富血小板血 (hPRP) 刺激小鼠和人类卵巢组织的休眠卵泡激活. 这一过程涉及卵细胞中的PI3K/Akt信号通路,为早产卵巢缺陷患者提供潜在的生育能力改善.
科学领域:
- 生殖生物学 生殖生物学
- 细胞信号传递 细胞信号传递
背景情况:
- 过早的卵巢衰竭 (POI) 给生育带来了挑战.
- 激活休眠原始毛囊是一种潜在的治疗策略.
- 人类富血小板血 (hPRP) 在恢复卵巢功能方面表现有前途,但机制尚不清楚.
研究的目的:
- 研究hPRP在原始毛囊激活中的作用和机制.
- 为了确定hPRP是否影响卵细胞中的PI3K/Akt信号通路.
主要方法:
- 用hPRP治疗培养的小鼠卵巢和人类卵巢组织.
- 评估了p-Akt和p-FOXO3a的蛋白质水平.
- 免疫光被用于可视化p-Akt.
- 使用LY294002.2,可以抑制PI3K/Akt通路.
主要成果:
- hPRP增加了小鼠卵巢中生长的毛囊数量和粒粉细胞的增殖.
- hPRP提高了p-Akt和p-FOXO3a蛋白水平,并诱导了卵细胞中的FOXO3a核出口.
- hPRP治疗增加了卵细胞中的p-Akt光强度.
- LY294002抑制了hPRP诱导的毛囊生长.
- 在体内和人类卵巢组织中,hPRP促进了卵泡生长和增加了p-Akt水平.
结论:
- 在小鼠和人类卵巢组织中,hPRP促进原始卵泡激活.
- 卵细胞中的PI3K/Akt信号通路调解hPRP的影响.
- hPRP代表了一种潜在的治疗剂,可以改善POI的生育能力.
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