相关实验视频

Updated: May 22, 2025

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Published on: May 21, 2020

多态短串重复的全基因组概况及其对不同水种群的基因表达和特征变异的影响

Xiyu Tan¹, Wanyong Zeng¹, Yujian Yang²

¹Guangdong Basic Research Center of Excellence for Precise Breeding of Future Crops, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Agriculture, South China Agricultural University, Guangzhou, Guangdong 510642, China.

Journal of genetics and genomics = Yi chuan xue bao

|March 15, 2025

概括

短串联重复 (STRs) 影响大米中的基因表达和特征. 这项研究绘制了米STR图,确定了表达STR (eSTR),并验证了它们的监管作用,为作物改进提供了新的目标.

科学领域:

基因组学就是基因组学.
植物生物学植物生物学
分子遗传学分子遗传学

背景情况:

众所周知,短串联重复 (STR) 影响基因表达和特征变异.
对STR基因组特征及其对大米基因表达的影响缺乏全面的理解.

研究的目的:

系统地描述大米基因组中的多态STR.
确定影响基因表达 (eSTRs) 的STR及其调节作用.
探索STRs在米遗传改进中的潜力.

主要方法:

构建一个多态STR地图,使用来自4726个米加入的种群规模再序列数据.
对STR发生,突变模式,分布和特性进行全基因组调查.
通过将STR长度变化与127个米加入中的基因表达相关联,使用转录组数据识别eSTR.
通过促进器编辑对STR功能的实验验证.

主要成果:

创建了137,629个多态STR的地图,揭示了STR特性和图案特征之间的相关性.
确定了44672个eSTR,主要位于活跃转录基因的调节区域.
人口分析揭示了具有遗传分歧的STR和1726个可能与农业特征相关的STR.
在OsFD1促进体中对STR的实验编辑证实了它在基因调节和表型中的作用.

结论:

STRs显著促进了植物的转录调节.
这项研究为利用STRs作为米遗传改进的目标提供了基础.
STRs代表了一种有价值的,未充分利用的基因组资源,用于了解植物变异和繁殖.

关键词:

基因表达基因表达基因表达基因组变异的发生大米大米大米大米大米在STR中,STR是STR.短串联重复重复的重复转录法规转录法规

更多相关视频

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Published on: September 2, 2019

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

相关概念视频

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

Pleiotropy

Pleiotropy

Pleiotropy is the phenomenon in which a single gene impacts multiple, seemingly unrelated phenotypic traits. For example, defects in the SOX10 gene cause Waardenburg Syndrome Type 4, or WS4, which can cause defects in pigmentation, hearing impairments, and an absence of intestinal contractions necessary for elimination. This diversity of phenotypes results from the expression pattern of SOX10 in early embryonic and fetal development. SOX10 is found in neural crest cells that form melanocytes,...

Genome-wide Association Studies-GWAS

Genome-wide Association Studies-GWAS

Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...