多omics集成和机器学习识别和验证中性粒细胞外细胞陷相关的基因特征在慢性鼻炎与鼻
Fu Shu1, Yaping Wang2, Linglong Li2
1College of Traditional Chinese Medicine, Chongqing Medical University, Chongqing 400000, People's Republic of China.
Clinical immunology (Orlando, Fla.)
|March 15, 2025
概括
研究人员确定了三种关键基因 (CXCR4,CYBB,PTAFR),这些基因与中性粒细胞外陷 (NETs) 相关,这些基因在具有鼻息肉的慢性鼻炎 (CRSwNP) 中起作用. 这些基因显示出作为CRSwNP的诊断生物标志物和治疗点的潜力.
科学领域:
- 免疫学 免疫学 免疫学
- 遗传学 是一个遗传学.
- 耳鼻喉科 耳鼻喉科 耳鼻喉科
背景情况:
- 带鼻息肉的慢性鼻炎 (CRSwNP) 是一种复杂的炎症上呼吸道疾病.
- 中性细胞外细胞陷 (NETs) 在CRSwNP的病原发生中起作用,但它们的分子基础需要进一步阐明.
研究的目的:
- 调查CRSwNP中NETs的分子特征.
- 根据NETs相关基因,确定CRSwNP的新型诊断生物标志物和治疗点.
主要方法:
- 使用差异表达基因分析,加权基因共同表达网络分析和机器学习算法.
- 用接收器操作特征 (ROC) 曲线分析和多中心数据验证来评估诊断性能.
- 进行了免疫透分析和单细胞RNA测序,以了解基因表达和细胞背景.
主要成果:
- 确定了三个核心NETs相关基因 (CXCR4,CYBB,PTAFR),并发现在CRSwNP患者中显著上调.
- 这些基因与中性粒细胞和免疫细胞透有很强的相关性.
- 单细胞RNA测序揭示了在髓状和免疫细胞中占主导地位的表达,在疾病进展过程中具有动态变化.
结论:
- 已确定CXCR4,CYBB和PTAFR为CRSwNP诊断的潜在新生物标志物.
- 这些基因可能通过IL-17信号传导和代谢途径促进CRSwNP的发病,这表明它们是个性化治疗的目标.
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