从咖啡副产品中优化联合的亚临界水和CO2提取,以提高咖啡副产品中的和抗氧化活性
Varunnarin Karprakhon1, Rinlada Sirisangsawang1, Kanidta Kaewkroek2
1Department of Chemical and Materials Engineering, Faculty of Engineering, Rajamangala University of Technology Thanyaburi, Pathum Thani, 12110, Thailand.
Scientific reports
|March 16, 2025
概括
这项研究优化了从咖啡副产品中提取化合物的亚临界水和CO2提取. 咖啡桃肉显示出比消耗咖啡粉更高的抗氧化活性,突出了其价值化潜力.
科学领域:
- 食品科学 食品科学 食品科学
- 绿色化学 绿色化学
- 生物技术是生物技术.
背景情况:
- 消耗的咖啡粉 (SCG) 和咖啡桃肉 (CCP) 是丰富的农业工业副产品.
- 这些副产品含有具有抗氧化特性的有价值的化合物.
- 需要有效的提取方法来利用这些咖啡残留物.
研究的目的:
- 为了优化从SCG和CCP中化合物的亚临界水提取 (SWE) 与高压二氧化碳 (CO2) 结合.
- 为了最大限度地提高总含量 (TPC) 和DPPH激素清除活动.
- 评估CCP和SCG作为生物活性化合物的来源的潜力.
主要方法:
- 使用设计专家V.13和中央复合设计 (CCD) 系统优化提取参数 (温度,时间,固体与水的比率).
- 使用DPPH测定提取化合物和评估抗氧化活性.
- 生物活性化合物的识别使用液体染色学-并联质谱学 (LC-MS/MS) 和薄层染色学 (TLC).
主要成果:
- 对SCG的最佳条件:198°C,60分钟,0.027g/mL固体与水的比率,TPC为217.26 mg GAE/g DW,DPPH为23.28 μMol TE/g DW.
- 对CCP的最佳条件: 189°C,54分钟,0.024g/mL固体/水比,产生230.13mg GAE/g DW的TPC和32.63μMol TE/g DW的DPPH.
- 与SCG相比,CCP表现出较高的TPC和抗氧化活性.
- LC-MS/MS和TLC证实了酸,神胺和咖啡因的存在.
结论:
- 亚临界水和CO2提取是一种有效的,无溶剂的方法,用于从咖啡副产品中回收生物活性化合物.
- 咖啡桃肉质是高价值化合物的有希望的来源.
- 优化的提取过程为食品,营养药品和制药行业提供了可持续的价值化机会.
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