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Updated: May 22, 2025

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通过微流体和定量建模研究TFEB核穿的动态调节

Iacopo Ruolo1,2,3, Sara Napolitano1,2,4, Lorena Postiglione1,2

  • 1Department of Chemical, Materials and Industrial Production Engineering, University of Naples Federico II, Naples, Italy.

Communications biology
|March 16, 2025
PubMed
概括

在营养缺乏期间,转录因子EB (TFEB) 的核转移发生在两个阶段,包括释放和mTORC1抑制. 重新养触发了通过出口素1的细胞质再定位,揭示了复杂的TFEB调节.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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