相关实验视频

Updated: Jun 13, 2026

Isolating Human Peripheral Blood Mononuclear Cells and CD4+ T cells from Sézary Syndrome Patients for Transcriptomic Profiling

Isolating Human Peripheral Blood Mononuclear Cells and CD4+ T cells from Sézary Syndrome Patients for Transcriptomic Profiling

Published on: October 14, 2021

延迟分离外围血液单核细胞对细胞活力和功能的影响

Sarah Lehle^1,2,3,4, Simon Völkl⁵, Katharina Seitz^1,2,3,4

¹Department of Gynecology and Obstetrics, Universitätsklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Erlangen, Germany.

|March 16, 2025

概括

延长血液保持时间会对外围血液单核细胞 (PBMC) 的质量产生负面影响,增加NK细胞的亡并降低功能. 尽量减少血液采集到PBMC隔离时间对于可靠的生物标志物测定至关重要.

科学领域:

免疫学免疫学免疫学
生物标志物发现发现
细胞生物学细胞生物学

背景情况:

周围血液单核细胞 (PBMC) 是免疫系统的关键生物标志物.
可靠的生物银行对于最小化多中心试验中的PBMC异质性至关重要.
延长血液输送时间可能会在隔离之前损害PBMC的完整性.

研究的目的:

为了评估延长血液保持时间对PBMC活力和细胞毒性的影响.
确定最佳的血液采集到隔离的时间表,以保持PBMC的质量.
评估对自然杀手 (NK) 和T细胞功能的影响.

主要方法:

从49名早期HER2阳性乳腺癌患者中,在采集后≤6小时或≥20小时内分离了PBMC.
使用自动细胞计数和流细胞计量,量化了PBMC产量,活力和细胞死亡.
分析了体外细胞毒性,NK细胞百分比,T细胞百分比,以及亡/亡细胞.

主要成果:

隔离PBMCs≥20小时后收集产生了更多的细胞,但降低了NK细胞的活力和强度解.
延长的保留时间显著降低了抗体介导的细胞毒性和抗体依赖的细胞.
虽然T和NK细胞的百分比没有受到影响,但PBMC≥20小时显示出更高的核突NK细胞百分比.

结论:

长时间的血液储存会增加NK细胞的亡和亡,损害PBMC的质量.
延长的保留时间会对NK细胞相关的功能和体外测试产生不利影响.
尽量减少血液保持时间对于保持PBMC完整性和NK细胞功能至关重要.

关键词:

这是生物银行.细胞活力细胞活力细胞毒性细胞毒性这就是PBMCs.第四阶段临床试验

更多相关视频

Isolation and Cryopreservation of Highly Viable Human Peripheral Blood Mononuclear Cells From Whole Blood: A Guide for Beginners

Isolation and Cryopreservation of Highly Viable Human Peripheral Blood Mononuclear Cells From Whole Blood: A Guide for Beginners

Published on: October 25, 2024

Author Spotlight: Advancements in PBMC Isolation – Enhancing Throughput and Consistency in Research

Author Spotlight: Advancements in PBMC Isolation – Enhancing Throughput and Consistency in Research

Published on: July 19, 2024

相关概念视频

Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.