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相关概念视频

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
3.2K

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相关实验视频

Updated: May 22, 2025

Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses
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Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses

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结冷保存细胞的转录学.

Y O Ciftci1, E Kaya2

  • 1Molecular Biology and Genetics Department; Smart Agriculture Research and Application Center; Central Research Laboratory (GTU-MAR), Faculty of Basic Sciences, Gebze Technical University, Kocaeli, Turkiye. ozden@gtu.edu.tr.

Cryo letters
|March 16, 2025
PubMed
概括
此摘要是机器生成的。

转录学,即对基因表达的研究,揭示了细胞如何在冷保存中存活. 这种方法有助于识别基因和通路,这些基因和通路对于细胞活力至关重要,并了解植物和哺乳动物的冷损伤.

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Working with Human Tissues for Translational Cancer Research
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相关实验视频

Last Updated: May 22, 2025

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科学领域:

  • 低温生物学 低温生物学
  • 分子生物学分子生物学
  • 基因组学就是基因组学.

背景情况:

  • 冷保存对于遗传资源的保护至关重要,但细胞应激机制尚不清楚.
  • 了解冷保存期间的分子调整对于提高生存能力至关重要.

研究的目的:

  • 审查最近的研究,将冷保存与转录生物学 (cryobiomics) 整合起来.
  • 评估优化哺乳动物和植物细胞冷保存的因素.
  • 探索转录学在理解冷损伤和寒冷耐受性方面的作用.

主要方法:

  • 使用转录学技术的科学文献的审查.
  • 在冷保存之前和之后对基因表达特征的分析.
  • 应用RT-PCR,实时PCR和RNA-seq.等方法.

主要成果:

  • 转录组学提供了冷保存细胞中基因表达变化的全球视图.
  • 识别基因,非编码RNA和影响细胞活力和增殖的途径.
  • 强调了转录学在理解冷损伤,发育障碍和植物耐寒性方面的实用性.

结论:

  • 转录学是了解细胞对冷保存的反应的强大工具.
  • 它有助于优化各种细胞类型的冷保存协议.
  • 使用转录学进行进一步的研究可以阐明植物中的冷回应机制.