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相关概念视频

Sample Handling01:02

Sample Handling

Transportation of samples from the collection point to the laboratory, as well as storage and preservation techniques, are crucial for maintaining sample integrity and ensuring accurate and reliable test results.
Samples should be transported carefully from collection points to the laboratory. They should be properly sealed and clearly labeled to prevent cross-contamination. To preserve the sample integrity, optimal temperature conditions during transport are essential. This could involve using...
Pasteurization and Food Preservation01:28

Pasteurization and Food Preservation

Pasteurization is a widely employed thermal processing technique designed to enhance the safety and shelf life of perishable food and beverages. By subjecting products to specific high temperatures for controlled durations, this method effectively inactivates pathogenic microorganisms and spoilage enzymes without significantly compromising sensory qualities. The technique has been pivotal in food safety management, especially for consumables susceptible to microbial contamination such as milk,...
Principles of Food Preservation01:27

Principles of Food Preservation

Food spoilage results from microbial growth, enzymatic activity, and environmental factors that gradually degrade the sensory, nutritional, and safety qualities of food. Preservation techniques aim to slow or halt these processes to extend shelf life and maintain product quality.A key concept in food microbiology is the microbial growth curve, which includes four phases: lag, exponential (log), stationary, and death. During the lag phase, bacteria adjust to their environment without significant...
Methods of Controlling Food Spoilage01:26

Methods of Controlling Food Spoilage

Food spoilage is caused by microbial growth or by chemical and physical changes, all of which affect the taste, texture, and safety of food.Temperature-Based PreservationRefrigeration at 0–4 °C slows microbial growth and enzyme activity, making it ideal for short-term storage. However, certain spoilage organisms—such as psychrotrophs like Listeria monocytogenes—can still proliferate at these temperatures. Freezing below -18 °C further slows biological processes by forming ice crystals, which...

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Cryopreservation of Preimplantation Embryos of Cattle, Sheep, and Goats
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牛花粉储存的冷保存协议

M Shankar1, R Gowthami2, K Tripathi2

  • 1Division of Plant Genetic Resources, ICAR-Indian Agricultural Research Institute, New Delhi-110012, India.

Cryo letters
|March 16, 2025
PubMed
概括

在液 (-196°C) 中冷保存牛花粉,可保持超过五个月的生命力,从而实现成功的受精. 这一突破对于牛 (Vigna unguiculata) 改善作物和杂交计划至关重要.

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科学领域:

  • 植物科学 植物科学
  • 农业学是一种农业学.
  • 低温生物学 低温生物学

背景情况:

  • 牛花粉的长期储存对于作物改善和广泛的杂交是必不可少的.
  • 有效的花粉保护有助于分离的母植物受精.

研究的目的:

  • 为了确定不同储存温度下牛花粉的寿命.
  • 为各种各样的牛加入开发一个冷保存协议.

主要方法:

  • 在长时间储存在4°C,-20°C和196°C后,评估了花粉活力.
  • 为了冷保存,确定了最佳的花粉水分含量 (12-14%) 和干燥时间 (5分钟).

主要成果:

  • 在4°C (1周) 和-20°C (2周) 时,花粉活力完全丧失.
  • 在 -196°C保存的花粉保持了可与新鲜花粉相比的活力超过5个月.
  • 冷保存的花粉成功地促进了正常受精.

结论:

  • 在196°C的冷保存为长期保存牛花粉提供了一种可行的方法.
  • 这种技术支持牛平分体生殖质的保护和广泛的杂交努力.