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基于肠道和肝脏代谢的Cuscutae精液中雌激素活性成分的逆可追溯性分析

Xiangming Sun¹, Mingyao Zhao¹, Wei Liang¹

¹School of Pharmacy, Harbin University of Commerce, Harbin 150076, China.

Journal of pharmaceutical and biomedical analysis

|March 16, 2025

概括

研究Cuscutae精液代谢以确定雌激素化合物. 研究人员确定了35种肝脏和3种肠道代谢物,澄清了代谢途径和潜在的活性成分,如kaempferol和quercetin.

科学领域:

药理学和中国传统医学
代谢学和质谱学.
内分泌学在内分泌学.

背景情况:

库斯库塔精液是一种传统的中医药,用于加强脏和调节雌激素.
之前的研究在库斯库塔的精液中发现了九种直接作用的雌激素物质.
这些物质的代谢途径和原型形式尚不清楚,阻碍了活性化合物的识别.

研究的目的:

为了阐明来自Cuscutae精液的九种直接作用的雌激素物质的体内代谢途径.
确定这些化合物的原型形式和代谢关系.
为库斯库塔种子的质量控制提供基础.

主要方法:

使用大鼠肝脏显微体和肠道菌群对肝脏和肠道代谢的研究.
超高性能液态色谱/四极飞行时间质谱 (UPLC/Q-TOFMS) 用于代谢物识别.
反向可追溯性分析以澄清代谢转化关系.

主要成果:

总共有35种肝脏代谢产物和3种肠道代谢产物被暂时确定.
肝脏新陈代谢主要由葡萄糖化.
肠道新陈代谢涉及类甘油酸的水解到甘油酸,没有进一步的II阶段反应.
已澄清了代谢转化关系,除了甲基化奎尔丁葡萄化物.

结论:

凯姆菲罗尔,奎尔塞丁,阿皮基宁,海培林,阿斯特拉加林和异基林是Cuscutae精液中潜在的雌激素活性物质.
这项研究澄清了代谢途径,并提供了关于Cuscutae精液有效成分的见解.
结果可以作为质量控制和进一步研究的参考.

关键词:

库斯库塔 (Cuscutae) 的精子是一种精子.肠道菌群是什么意思肠道菌群肝脏显微体是什么意思代谢产物的代谢物反向可追溯性分析的反向可追溯性分析

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