抗生素塑造了内源性部分脱系统中花和生物膜之间的核心微生物社区分布:从代谢途径的洞察力
Kai-Yue Dong1, Chao-Xi Yang1, Jin-Luo Pang1
1School of Engineering, Hangzhou Normal University, Hangzhou 311121, PR China.
Water research
|March 16, 2025
概括
抗生素如硫法 (SDZ) 和西普洛素 (CIP) 通过促进微生物能量代谢和促进有益细菌迁移到生物膜来加速部分脱 (PD),从而增强酸盐的去除. 这项研究揭示了微生物群落如何适应废水处理系统中的抗生素压力.
科学领域:
- 环境微生物学环境微生物学
- 污水处理 污水处理 污水处理
- 生物地质化学循环是什么
背景情况:
- 在抗生素压力下的部分脱 (PD) 系统中的微生物反应机制,特别是关于能量代谢和电子转移的机制,尚不清楚.
- 这种知识差距限制了将微生物动态与废水处理中的反应堆性能联系起来的能力.
- 了解这些反应对于优化药物暴露环境中的PD过程至关重要.
研究的目的:
- 研究在硫法迪亚 (SDZ) 和西普罗夫洛克萨 (CIP) 压力下移动床生物膜反应器 (MBBR) 内微生物群体和新陈代谢的动态变化.
- 阐明微生物能量代谢,电子转移和社区结构在抗生素暴露期间维持PD性能方面的作用.
- 为了解PD微生物联盟对抗生素污染的适应机制提供理论基础.
主要方法:
- 利用移动床生物膜反应器 (MBBR) 在受控条件下模拟部分脱 (PD).
- 引入不同度 (2 mg/L和 4 mg/L) 的硫法迪亚 (SDZ) 和西普洛素 (CIP),以评估抗生素应激.
- 采用基因组测序和关键功能基因 (例如 sucC, PK, narGHI, IDH1, DLD, DLAT) 的分析来评估微生物社区结构和代谢途径.
主要成果:
- 剂量为2 mg/L的SDZ或CIP加速了酸盐积累15天,并保持了高的酸盐去除效率 (83.5-93.9%).
- 抗生素压力促进了三酸循环和糖解,导致NADH和ATP的增加,从而加速了代谢.
- 较高的抗生素度 (4 mg/L) 诱导了Thauera属从向生物膜的选择性迁移,其中narGHI和电子传输基因更丰富,确保稳定的PD性能.
结论:
- 微生物的能量代谢和电子转移途径被SDZ和CIP显著改变,有利于PD系统中早期酸盐的积累.
- 像Thauera这样的关键细菌迁移到生物膜,再加上生物膜中特定功能基因的丰富性增加,对于在抗生素压力下保持稳定的部分脱是至关重要的.
- 这些发现提供了微生物联盟在处理面临制药污染的废水处理中的弹性和适应策略的见解.
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