相关实验视频

Updated: May 22, 2025

In Situ Hybridization for the Precise Localization of Transcripts in Plants

In Situ Hybridization for the Precise Localization of Transcripts in Plants

Published on: November 23, 2011

在位固定 (ISF):一种快速,可重复使用和高通量核酸提取方法,用于植物分子分析

Lu Chen¹, Qiang Li², Guorun Qu²

¹Yazhou Bay Institute of Deepsea Sci-Tech, Shanghai Jiao Tong University, Sanya, 572024, PR China; Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, PR China.

Biosensors & bioelectronics

|March 16, 2025

概括

一种新的 in-situ 固定 (ISF) 方法可以在不磨碎的情况下,从植物叶子中快速提取6分钟的核酸. 这简化了用于育种和诊断的植物分子分析,提供了可在现场部署的解决方案.

科学领域:

农业科学农业科学
分子生物学分子生物学
生物技术是生物技术.

背景情况:

核酸测试 (NAT) 对植物育种和作物保护至关重要.
现有的植物DNA/RNA提取方法通常是缓慢的,复杂的,不适合于点的护理应用.
植物细胞的复杂性给有效的核酸隔离带来了挑战.

研究的目的:

为植物开发一种快速,高吞吐量和简化的核酸提取方法.
为了能够在现场进行经济高效的植物分子分析.
为了实际应用,将该方法与各种NAT技术集成在一起.

主要方法:

开发了一种用于植物核酸提取的新型现场固定 (ISF) 方法.
消除了研磨,离心和管管等步骤.
用聚合酶链反应 (PCR),定量PCR (qPCR) 和循环介导同热放大 (LAMP) 验证的ISF.

主要成果:

在6分钟内实现了多达96个样本的核酸提取.
已证明ISF提取的核酸与PCR,qPCR和LAMP的兼容性.
成功应用ISF用于检测转基因内容,植物病原体和米杂交基因定型.
展示了试剂的可重复使用性,没有交叉污染.

结论:

ISF方法为植物核酸提取提供了一个简单,快速和高吞吐量解决方案.
ISF适用于各种应用,包括基因定型,转基因检测和病原体诊断.
该方法与可在现场部署的同热放大技术的兼容性使得现场植物分子分析更加容易.

关键词:

高通量分析的分析.在现场固定固定.分子诊断学分子诊断学核酸提取方法核酸提取方法植物叶子植物叶子

更多相关视频

Rapid In Vivo Fixation and Isolation of Translational Complexes from Eukaryotic Cells

Rapid In Vivo Fixation and Isolation of Translational Complexes from Eukaryotic Cells

Published on: December 25, 2021

Genotyping of Plant and Animal Samples without Prior DNA Purification

Genotyping of Plant and Animal Samples without Prior DNA Purification

Published on: September 24, 2012

相关概念视频

In-situ Hybridization

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...

DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...