分析N,N-二甲基三胺 (DMT) 和其代谢物,使用LC-MS/MS用于法医目的
Munchelou M Gomonit1, Madeleine J Swortwood1,2, Michael T Truver3
1Department of Forensic Science, College of Criminal Justice, Sam Houston State University, Huntsville, Texas, USA.
Journal of forensic sciences
|March 16, 2025
概括
这项研究开发了一种检测N,N-二甲基胺 (DMT) 和其代谢物N,N-二甲基胺-N-氧化物 (DMT-NO) 在人体样本中的方法. 这些生物标志物可以帮助确定最近在法医案件中使用的DMT.
科学领域:
- 药理学和毒理学 药理学和毒理学
- 分析化学 分析化学
- 法医科学 法医科学 法医科学
背景情况:
- 阿雅华斯卡含有N,N-二甲基三胺 (DMT),是一种迷幻化合物.
- DMT被代谢成英多尔-3-酸 (IAA) 和N,N-二甲基胺-N-氧化物 (DMT-NO).
- 准确量化DMT及其代谢物对于法医调查至关重要.
研究的目的:
- 开发和验证一种敏感的LC-MS/MS方法,用于在人体血和尿液中量化DMT,DMT-NO和IAA.
- 评估DMT和DMT-NO作为检测外源DMT消费的生物标志物的适用性.
主要方法:
- 开发并验证了在血和尿液中检测DMT,DMT-NO和IAA的LC-MS/MS试验.
- 使用的蛋白质沉与乙二:甲醇用于样本制备.
- 根据ANSI/ASB 036建议评估方法性能,包括线性,精度,偏差和稳定性.
主要成果:
- 经过验证的方法显示了高的分析物回收率 (≥91%) 和可接受的精度 (≤6.4%) 和偏差 (±17.5%).
- 对血中的DMT,DMT-NO和IAA以及尿中的DMT和DMT-NO建立了线性范围.
- 概念验证研究显示,与血相比,DMT和DMT-NO的尿度更高,表明新陈代谢和清除速度快.
结论:
- DMT和DMT-NO是用于法医识别DMT使用的可靠和特定的生物标志物.
- 由于其内源性存在,不建议使用印醇-3-酸 (IAA) 作为唯一的生物标志物.
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