相关实验视频

Updated: May 22, 2025

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response

Published on: September 15, 2017

斑块蛋白不参与由cAMP调节的内皮屏障稳定

Ibrahim Hamad¹, Sara Sepic¹, Sina Moztarzadeh¹

¹Chair of Vegetative Anatomy, Institute of Anatomy, Faculty of Medicine, Ludwig-Maximilians-University (LMU), Pettenkoferstraße 11, 80336, Munich, Germany.

Scientific reports

|March 17, 2025

概括

缺少斑块球蛋白 (PG) 通过增加结节蛋白来增强内皮屏障功能. 虽然cAMP在两种细胞类型中增强了屏障完整性,但PG缺乏减少了这种效应,突出了PG.

科学领域:

内皮细胞生物学内皮细胞生物学
蜂信号传输是如何进行的
血管的通透性血管的通透性

背景情况:

内皮屏障的完整性对血管健康至关重要,由actin细胞骨架重塑和细胞结合蛋白调节.
斑块球蛋白 (PG) 是细胞粘附和信号的关键蛋白质,与cAMP和Rho GTPases等分子一起影响血管透性.

研究的目的:

研究斑块球蛋白 (PG) 在cAMP介导的内皮屏障稳定中的作用.
为了比较野生型 (WT) 和PG敲除型 (PG-KO) 心肌内皮细胞的内皮屏障功能.

主要方法:

从WT和PG-KO小鼠中建立心肌内皮细胞.
使用超内皮电阻 (TEER) 测量内皮屏障功能的测量.
对结点蛋白表达 (VE-cadherin,β-catenin,PECAM-1),酸酶活性 (VE-PTP) 和Rho GTPase活性 (Rac1,RhoA) 的分析.

主要成果:

PG-KO细胞表现出增加的基底屏障功能与增强的VE-cadherin和β-catenin在结点.
PG缺乏减少了基底细胞内cAMP水平,但没有改变VE-cadherin/β-catenin复合物的组成或Rac1/RhoA活性.
在两种细胞类型中,cAMP增强改善了屏障功能和Rac1活性,尽管在PG-KO细胞中效果较差.

结论:

斑块球蛋白在调节基底内皮屏障功能方面发挥作用,并调节对cAMP介导的屏障稳定反应.
缺少PG会导致内皮屏障本质上更紧,但会损害细胞在对cAMP的反应中进一步增强的能力.

更多相关视频

A Cell Culture Model of Resistance Arteries

A Cell Culture Model of Resistance Arteries

Published on: September 8, 2017

Study of the Actin Cytoskeleton in Live Endothelial Cells Expressing GFP-Actin

Study of the Actin Cytoskeleton in Live Endothelial Cells Expressing GFP-Actin

Published on: November 18, 2011

相关概念视频

Cytoskeletal Linker Proteins - Plakins

Cytoskeletal Linker Proteins - Plakins

Plakins are large proteins with binding domains for microtubules, microfilaments, intermediate filaments, and membrane-associated protein complexes at cell junctions. Plakin functions are evolutionarily conserved and are primarily involved in organizing the different components of the cytoskeleton by crosslinking them to each other and connecting them to the cell-matrix and cell adhesion complexes. They are also known to interact with signal transducers, serve as scaffolds for signaling...

cAMP-dependent Protein Kinase Pathways

cAMP-dependent Protein Kinase Pathways

Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...

Formation of the Platelet Plug

Formation of the Platelet Plug

The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...

Intracellular Signaling Affects Focal Adhesions

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...

The JAK-STAT Signaling Pathway

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...