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Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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Mutations01:35

Mutations

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Mutations are changes in the sequence of DNA. These changes can occur spontaneously or they can be induced by exposure to environmental factors. Mutations can be characterized in a number of different ways: whether and how they alter the amino acid sequence of the protein, whether they occur over a small or large area of DNA, and whether they occur in somatic cells or germline cells.
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...
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Updated: May 22, 2025

Photopatterning Proteins and Cells in Aqueous Environment Using TiO2 Photocatalysis
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Photopatterning Proteins and Cells in Aqueous Environment Using TiO2 Photocatalysis

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紫外线辐射的透深度在TiO2上

Bugrahan Guner1, Mohammad Safikhani-Mahmoudi1, Fengmiao Li2,3

  • 1Department of Mechanical Engineering, École de technologie supérieure, University of Quebec, Montreal, QC, Canada.

Communications chemistry
|March 17, 2025
PubMed
概括

高能紫外线 (UVC) 辐射会在金属氧化物 (MO) 中产生缺陷,如TiO2. 我们的研究表明,紫外线可穿透MO数百微米,远远深于此前所认为的.

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科学领域:

  • 材料科学 材料科学 材料科学
  • 表面科学是一门学科.
  • 摄影化学的使用.

背景情况:

  • 金属氧化物 (MO) 的高能紫外线 (UVC) 辐射会诱导光诱导的表面氧空缺 (PI-SOV).
  • PI-SOVs显著改变了MO的电子和化学特性,影响了电荷载体的动态.
  • 在MO中,UVC辐射的透深度是设备应用的关键参数,但人们对其了解甚少.

研究的目的:

  • 为了研究和量化UVC辐射在金属氧化物中的透深度.
  • 为了确定UVC辐射对表面之外的散装物质的影响程度.
  • 为设计和运行利用紫外线的基于MO的系统提供关键数据.

主要方法:

  • 在背面照明的二氧化 (TiO2) 样本上进行光学传输测量.
  • 时间解析原子力显微镜 (TR-AFM) 用于观察辐射诱导的变化.
  • 在大部分MO样本中对UVC诱导的影响进行实验分析.

主要成果:

  • 观测到的UVC辐射效应是数百微米的TiO2样本中的大部分.
  • 这种透深度是大小的数量级大于以前假设的值.
  • 光诱导的表面氧空缺 (PI-SOV) 和它们的影响在很大程度上延伸到材料中.

结论:

  • 紫外线辐射在金属氧化物中的透深度远远大于以前的假设.
  • 这些发现需要对UVC与MO相互作用模型进行重新评估.
  • 这项研究对基本理解和实际设备工程有重大影响.