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工程益生菌使得有针对性的肠道输送双重气体传递剂可用于炎症性肠病治疗
Tengfei Ma1, Guihai Gan1, Jian Cheng1
1Department of Pharmacy, The First Affiliated Hospital of University of Science and Technology of China (USTC), Division of Life Sciences and Medicine, and State Key Laboratory of Precision and Intelligent Chemistry, University of Science and Technology of China, Hefei, Anhui Province, 230026, China.
工程益生菌将治疗气体一氧化碳 (CO) 和硫化 (H2S) 直接输送到肠道,有效治疗小鼠的炎症性肠病 (IBD) 和相关焦虑和抑郁症.
科学领域:
- 胃肠道学和免疫学
- 微生物组治疗学 微生物组治疗学
- 药物输送系统 药物输送系统
背景情况:
- 炎症性肠病 (IBD) 是一种慢性疾病,严重影响患者的生活质量,经常与焦虑和抑郁症同时发生.
- 内源性气体传递物如一氧化碳 (CO) 和硫化 (H2S) 具有抗炎性质,但面临传递挑战.
- 针对性地向炎症肠道输送治疗剂仍然是IBD管理的关键未满足需求.
研究的目的:
- 开发一种新的策略,利用工程益生菌,针对肠道输送CO和H2S.
- 在IBD小鼠模型中评估这种方法的疗效,重点关注炎症,肠道屏障功能和微生物群调节.
- 评估向气传递剂在IBD中对神经炎症和相关行为症状的影响.
主要方法:
- 工程大肠杆菌尼斯尔1917 (EcN) 携带释放CO/H2S的共聚物 (POSR@EcN).
- 通过POSR@EcN in vivo评估增强的肠道定居和局部释放的CO/H2S.
- 分析POSR@EcN治疗对肠道炎症,屏障完整性,肠道微生物群组成和短链脂肪酸生产的影响.
- 在接受POSR@EcN治疗的IBD小鼠中评估神经炎症和焦虑/抑郁类行为.
主要成果:
- POSR@EcN证明了增强的肠道定居,以及在炎症的肠道组织中控制,局部释放的CO/H2S.
- 用POSR@EcN治疗有效调节炎症,恢复肠道屏障的完整性,并促进更健康的肠道微生物群.
- 通过POSR@EcN对CO/H2S的向输送增加了神经保护性代谢物,减少了神经炎症,并减轻了IBD小鼠的焦虑和抑郁类行为.
- 设计的益生菌策略成功地减轻了IBD关键症状和相关的心理并发症.
结论:
- 工程 EcN 作为一种有效的益生菌载体,用于向肠道输送气体传递物 (CO/H2S).
- 这种新的方法为管理炎症性肠病及其相关的心理健康问题提供了一个有前途的治疗策略.
- 通过工程益生菌提供有针对性的气传递物提供了IBD治疗的潜在突破,解决了胃肠道和神经系统症状.
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