主要成分,代谢和微生物特征的景观,以及它们在Eucommiae Cortex堆"出汗"期间的相关性
Linfeng Wang1,2, Mengxian Wu1,2, Bingnan Gu1,2
1School of Life Sciences, Institute of Microbial Engineering, Henan University, Kaifeng, China.
Frontiers in microbiology
|March 17, 2025
概括
传统的Eucommiae皮层 (EC) 的"出汗"过程增强了关键的活性化合物,并改变了代谢物和微生物群落. 这项研究揭示了在加工过程中EC质量的微生物调节,有助于质量控制.
科学领域:
- 药理学是指药理学,它是指药理学.
- 微生物学 微生物学
- 代谢学 代谢学 代谢学
背景情况:
- 皮质 (EC) 质量受到传统加工方法的影响,如"出汗".
- "出汗"如何影响EC的药用性质的确切机制尚不清楚.
研究的目的:
- 研究在EC"出汗"过程中活性成分,代谢物和细菌群体的动态变化.
- 阐明在这种传统加工方法中对EC质量的微生物调节.
主要方法:
- 使用了高性能液态染色学 (HPLC) 和基于UPLC/MS的非向代谢学.
- 高通量测序用于分析细菌群落.
- 在传统的堆叠"出汗"方法中,每天收集样品,持续5天.
主要成果:
- 关键的活性成分 (基尼酸,酸,素,皮诺雷西诺二甲糖化物,全方位黄酸) 在蒸后显著增加.
- 鉴定了807种不同的代谢物,并观察到微生物多样性的显著变化.
- 确定了像*Gluconobacter*和*Pseudomonas*这样的核心功能性微生物,与二次代谢产品的变化有关.
结论:
- "出汗"过程显著影响EC的活性化合物,代谢物和微生物组成.
- 一个相关性网络突出显示了在加工过程中EC质量的微生物调节.
- 研究结果支持优化处理时间和微生物管理,以加强质量控制.
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