相关实验视频

Updated: May 22, 2025

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion
13:43

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

Published on: January 31, 2022

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解卷以恢复具有异型分辨率的冷电磁图.

Yifan Cheng1, Junrui Li2, Yifei Chen2

  • 1HHMI/University of California San Francisco.

Research square
|March 17, 2025
PubMed
概括

单颗粒冷电子显微镜 (cryo-EM) 可能会受到偏好的方向的影响,导致异型分辨率. 一种新的解卷法,AR-Decon,从计算上改善了这些数据集的3D地图质量.

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Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

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相关概念视频

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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