Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

3.2K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
3.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Antibody reveals conformational latch regulating dimerization in β- and γ-herpesvirus proteases.

Nature communications·2026
Same author

CryoROLE: describing large inter-domain rotation in single particle cryo-EM.

bioRxiv : the preprint server for biology·2026
Same author

Nano-Silver-Selenium Liquid Dressing Facilitates Treatment of Monkeypox and Prevention of Viral Transmission in a Surrogate Mouse Model.

Exploration (Beijing, China)·2026
Same author

Facet-Dependent Water Inhibition of Alkanol Dehydration on TiO<sub>2</sub> via Distinct Water-Alkanol Complexes.

Angewandte Chemie (International ed. in English)·2026
Same author

Characterization Standard for <i>In-situ</i> Cryo-electron Tomography.

bioRxiv : the preprint server for biology·2026
Same author

Structural characterization of kappa-opioid receptor dimer in complex with two G proteins.

Nature communications·2026

相关实验视频

Updated: May 22, 2025

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion
13:43

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

Published on: January 31, 2022

12.9K

解卷以恢复具有异型分辨率的冷电磁图.

Yifan Cheng1, Junrui Li2, Yifei Chen2

  • 1HHMI/University of California San Francisco.

Research square
|March 17, 2025
PubMed
概括

单颗粒冷电子显微镜 (cryo-EM) 可能会受到偏好的方向的影响,导致异型分辨率. 一种新的解卷法,AR-Decon,从计算上改善了这些数据集的3D地图质量.

更多相关视频

Single Particle Cryo-Electron Microscopy: From Sample to Structure
11:52

Single Particle Cryo-Electron Microscopy: From Sample to Structure

Published on: May 29, 2021

8.3K
Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope
09:49

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Published on: March 16, 2022

5.0K

相关实验视频

Last Updated: May 22, 2025

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion
13:43

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

Published on: January 31, 2022

12.9K
Single Particle Cryo-Electron Microscopy: From Sample to Structure
11:52

Single Particle Cryo-Electron Microscopy: From Sample to Structure

Published on: May 29, 2021

8.3K
Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope
09:49

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Published on: March 16, 2022

5.0K

科学领域:

  • 结构生物学是结构生物学.
  • 生物物理学的生物物理.
  • 生物化学 生物化学

背景情况:

  • 单颗粒冷电子显微镜 (cryo-EM) 是确定高分辨率结构的强大工具.
  • 精确的3D结构确定依赖于随机定向的粒子.
  • 偏好的粒子方向导致异型分辨率,限制结构精度.

研究的目的:

  • 开发一种计算方法来处理冷EM数据中的异构分辨率.
  • 为了提高3D地图的质量,从偏好的方向的数据集中重建3D地图.

主要方法:

  • 建立了一个名为AR-Decon.的解卷方法.
  • 经过测试和验证的AR-Decon与合成和实验冷EM数据集.
  • 将AR-Decon的性能与基于机器学习的替代方法进行比较.

主要成果:

  • 在计算上,AR-Decon提高了具有异构分辨率的3D地图的质量.
  • 该方法有效地纠正由偏好的粒子方向引起的工件.
  • 验证证明了AR-Decon在不同数据集上的稳定性.

结论:

  • AR-Decon提供了一种可行的计算解决方案,用于提高冷EM地图质量.
  • 这种方法可以帮助克服结构生物学中偏好的导向挑战.
  • 通过使用冷EM,AR-Decon有助于更准确和可靠的结构确定.