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在酵母中确定短串联重复序列和基因表达之间的关联,揭示了编码转录调节蛋白的特定重复动机

Zongyuan Yu^1,2, Yating Liang², Meida Xiang²

¹School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China.

Computational and structural biotechnology journal

|March 17, 2025

概括

酵母 (Saccharomyces cerevisiae) 的编码序列中的短串重复 (STR) 与较低的基因表达有关. 这些重复的元素会影响染色质结构,并且在调节转录的基因中得到丰富.

科学领域:

基因组学就是基因组学.
分子生物学分子生物学
酵母遗传学酵母遗传学

背景情况:

串联重复序列 (TRs) 是在编码和非编码区域中发现的重复基因组元素.
虽然已知酵母促进体中的TR作用,但它们在编码序列 (CDS) 中的功能不明.
麦芽是一种基因研究的关键模型生物.

研究的目的:

研究Saccharomyces cerevisiae的CDS中的TR类型,分布和影响.
探索CDS中的TRs与基因表达水平之间的关系.
分析酵母CDS中TRs的表观遗传特征和功能丰富.

主要方法:

集成多个高吞吐量数据集:RNA-seq,ChIP-seq,ATAC-seq,Hi-C和微C.
在酵母CDS中分析TR分布和类型.
TR存在与基因表达,基因组修饰,染色质可访问性和相互作用的相关性.

主要成果:

在它们的CDS中具有短串联重复 (STR) 的基因表达水平明显较低.
含有STR的CDS区域表现出压制性组织蛋白标记,减少染色质可访问性和较少的染色质相互作用.
特定的三核酸和六核酸STR基因在编码转录调节蛋白的基因中得到丰富.

结论:

在Saccharomyces cerevisiae的CDS中的STRs影响基因表达和染色体调节.
STRs在涉及转录调节的基因中特别普遍.
识别的STR动机可以作为设计新型转录调节元件的目标.

关键词:

编码序列的编码序列.表观遗传特征是一种表观遗传特征.这种植物是Saccharomyces cerevisiae.短串联重复 (STRs) 是一个简单的重复.转录法规转录法规

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