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远大脑发育中的外部亡和亡:单细胞质量细胞计研究

Jiachen Shi, Weile Liu, Alison Song

bioRxiv : the preprint server for biology

|March 17, 2025

概括

调节的细胞死亡塑造了发育中的大脑. 联合删除RIPK3和Caspase-8增加了细胞数量,揭示了亡和亡在大脑发育中的不同作用.

科学领域:

神经科学是一个神经科学.
发育生物学发展生物学
细胞死亡研究细胞死亡研究

背景情况:

调节细胞死亡对大脑发育至关重要.
在这个过程中,外部亡和亡的具体作用尚未完全理解.

研究的目的:

调查RIPK3介导的亡和Caspase-8介导的亡对telencephalon发育的贡献.
在缺乏这些细胞死亡途径的小鼠中描述细胞格局.

主要方法:

使用单细胞质量细胞计 (CyTOF).
对野生类型 (WT),RIPK3淘汰 (RIPK3 KO) 和RIPK3/Caspase-8双淘汰 (DKO) 鼠标telencephalons进行分析.

主要成果:

联合删除RIPK3和Caspase-8导致总细胞数显著增加12.6%.
DKO小鼠显示Tbr2+中间基因和内皮细胞的选择性丰富.
这挑战了只有内在亡才能控制发育细胞淘汰的观念.

结论:

外在的亡和亡途径在远脑发育中具有不同的,特定于细胞类型的作用.
这些发现为发育中的大脑细胞数量调节提供了新的视角.
这项研究表明,与神经发育障碍的潜在联系包括异常细胞死亡.

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