相关实验视频

Updated: May 22, 2025

Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique
06:32

Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique

Published on: March 9, 2022

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H2A.Z通过促进DNA和海斯顿H3尾部的移动性来促进Sox2-核酶相互作用

Helen K Moos, Rutika Patel, Sophie K Flaherty

    bioRxiv : the preprint server for biology
    |March 17, 2025
    PubMed
    概括

    基因组变异H2A.Z通过增加DNA可访问性和减少基因组H3尾巴的竞争来增强先驱因子结合. 这种表观遗传机制对于启动细胞命运特异性基因表达至关重要.

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    Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark10:09

    Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

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    Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA10:40

    Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

    Published on: September 10, 2013

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    相关概念视频

    The Nucleosome Core Particle01:12

    The Nucleosome Core Particle

    Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
    Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...
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    The Nucleosome01:19

    The Nucleosome

    Human DNA is almost two meters long. However, it is compressed inside a tiny nucleus measuring only a few microns in diameter. To make this degree of compaction possible, DNA is organized into several sequential levels so that it can fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
    In a chromosome, DNA is wound twice around a protein complex called a histone octamer core, which consists of 8 histone proteins. This...
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    Nucleosome Remodeling02:54

    Nucleosome Remodeling

    Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
    Nucleosome remodeling complex
    Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
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    Histone Modification02:32

    Histone Modification

    The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
    Acetylation
    The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
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    Heterochromatin02:38

    Heterochromatin

    The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
    Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
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    Spreading of Chromatin Modifications02:25

    Spreading of Chromatin Modifications

    The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
    Writers
    The writer...
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