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细菌性阴道炎毒素会损害精子容量和受精
bioRxiv : the preprint server for biology
|March 17, 2025
概括
细菌性阴道炎毒素,脂多糖 (LPS) 和阴道素 (VLY),损害精子功能. 这些毒素会破坏精子的运动和受精能力,导致不孕.
科学领域:
- 生殖生物学 生殖生物学
- 微生物学 微生物学
- 毒理学 毒理学 毒理学
背景情况:
- 细菌性阴道炎 (BV) 与不孕症有关,涉及过度生长的产生毒素的细菌,如脂多糖 (LPS) 和阴道素 (VLY).
- 这些BV相关毒素对精子功能及其对不孕症的贡献的具体影响仍然不完全理解.
研究的目的:
- 研究与细菌性阴道炎 (BV) 相关的毒素,特别是脂多糖 (LPS) 和阴道素 (VLY) 对人类和小鼠精子功能的影响.
- 阐明这些毒素影响精子运动,电容,体外细胞形成和细胞内水平的机制.
主要方法:
- 人类和小鼠的精子在培养和非培养条件下暴露于LPS和VLY.
- 用计算机辅助的精子分析和光染色来评估精子的运动性,过度激活,活力和体外细胞形成.
- 用Fluo-4 AM染色和光成像测量细胞内动态.
- 研究了托尔类受体4 (TLR4) 和CatSper通道在LPS诱导影响中的作用.
主要成果:
- 暴露于LPS和VLY显著降低了小鼠精子中的体外受精率.
- 这两种毒素最初都增加了,然后在小鼠和人类的精子中显著降低了精子的运动性,过度活化和体外细胞形成.
- 这些效应与细胞内的快速,不可逆转的增加有关,这依赖于和TLR4介导的LPS.
结论:
- 与BV相关的毒素,LPS和VLY,显著损害精子功能,包括运动性,容量和受精卵细胞的受精能力.
- 这些毒素通过破坏关键的精子过程而导致不孕,突出了将BV与降低生育能力联系在一起的潜在机制.
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