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线粒体功能障碍在口腔致癌症:从皮细胞学的见解

R Keerthika¹, Jadhav Sachin Kumar², Akhilesh Chandra¹

¹Unit of Oral Pathology and Microbiology, Faculty of Dental Sciences, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221005 Uttar Pradesh India.

Indian journal of otolaryngology and head and neck surgery : official publication of the Association of Otolaryngologists of India

|March 17, 2025

概括

通过Janus Green B染色评估的线粒体功能障碍,随着口腔潜在恶性疾病 (OPMDs) 和口腔状细胞癌 (OSCC) 的出现而增加. 这种功能障碍与吸烟有关,可以预测恶性转变风险.

科学领域:

口腔病理学口腔病理学
线粒体生物学线粒体生物学
致癌的发生是致癌的产生.

背景情况:

口腔状细胞癌 (OSCC) 是一个重要的全球健康问题,通常由口腔潜在恶性疾病 (OPMDs) 先行.
线粒体功能障碍越来越被认为是癌症发展和进展的关键因素.
了解线粒体功能障碍在OPMD和OSCC中的作用对于早期检测和治疗至关重要.

研究的目的:

为了研究OPMD和OSCC中的线粒体功能障碍,使用Janus Green B (JGB) 染色.
为了将线粒体功能障碍与烟草暴露相关联.
评估JGB染色作为恶性转变和攻击性的生物标记物的潜力.

主要方法:

皮细胞学用于从正常,OPMD和OSCC样本中收集口腔涂抹.
应用了Janus Green B (JGB) 染色来评估线粒体活动.
染色强度和阳性细胞的百分比被量化并与临床病理学参数和烟草使用相关联.

主要成果:

观察到JGB染色强度和阳性细胞的百分比逐渐增加,从正常组织到OPMD,再到OSCC.
在中度至高度的JGB染色和烟草使用频率/持续时间之间发现了显著的相关性.
确定了JGB阳性细胞的特定切断值,以区分高风险OPMD并预测OSCC转化.

结论:

线粒体功能障碍是口腔致癌的一个关键事件,其严重程度从OPMD增加到OSCC.
JGB染色作为线粒体功能障碍的可靠指标,有助于评估OPMD恶性转变风险和OSCC的攻击性.
烟草使用是线粒体功能障碍在口腔癌的发展和进展中的重要贡献者.

关键词:

雅努斯绿色B染色方式线粒体功能障碍线粒体功能障碍口腔致癌的发生.口腔潜在的恶性疾病.口腔状细胞癌的癌症.

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