在Mycobacterium avium ssp.中的刻板印象免疫反应. 在不同的瑞士山羊基因型中感染结核病
Melina Rasper-Hössinger1, Simone Scherrer1, Roger Stephan1
1University of Zurich, Zurich, Switzerland.
Veterinary pathology
|March 17, 2025
概括
羊群中的结核病是由Mycobacterium avium ssp.引起的 准结核病 (MAP) C型和S型菌株. 虽然肠道病变没有显著差异,但S型菌株在淋巴结中引起了更多的炎症,这表明疾病的进展有所不同.
科学领域:
- 兽医病理学 兽医病理学
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
背景情况:
- 结核病是由Mycobacterium avium ssp.引起的 甲状腺结核病 (MAP),导致反动物的慢性肠炎.
- 在山羊中,MAP感染涉及C (牛) 型和S (羊) 型菌株.
- 了解应变损伤相关性对于管理山羊甲结核病至关重要.
研究的目的:
- 调查MAP菌株 (C型和S型) 与瑞士山羊组织形态病变之间的相关性.
- 分析炎症和亡标志物的免疫组织化学表达.
- 为了比较不同MAP菌株之间的病变发展和炎症细胞透.
主要方法:
- 使用PCR和SNP分析对10个瑞士山羊MAP分离物的表征.
- 菌株间隔的重复单元和变数串联重复 (MIRU-VNTR) 分析.
- 组织学评分,免疫组织化学 (CD3,CD79a,Iba1,切割caspase3,IL-17) 和肠道和介质淋巴结组织的形态分析.
主要成果:
- 在C型和S型MAP菌株之间没有发现肠道组织学损伤得分的显著差异.
- 在两组的肠道粘膜中观察到类似的炎症和亡细胞数量.
- S型MAP感染与中腔淋巴结中Iba1-和分裂的卡斯巴酶3阳性细胞数量显著增加有关.
结论:
- 马普C型和S型菌株在瑞士山羊中引发了类似的肠病变.
- 中腔淋巴结炎症在S型和C型感染之间有所不同,可能表明不同的致病途径.
- 需要进一步研究MAP病原和宿主-寄生虫相互作用.
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