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ZmDIR5的突变降低了玉米对浸水,盐度和干旱的耐受性

Zhixiong Zhao¹, Tao Qin¹, Hongjian Zheng¹

¹Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai Engineering Research Center of Specialty Maize, Crop Breeding and Cultivation Research Institution/CIMMYT-China Specialty Maize Research Center, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.

Plants (Basel, Switzerland)

|March 17, 2025

概括

导体 (DIR) 基因ZmDIR5积极调节玉米对浸水,盐和干旱压力的耐受性. 突变者表现出增长减缓和应激反应受损,突出显示ZmDIR5

科学领域:

植物分子生物学植物分子生物学
植物生理学植物生理学
遗传学遗传学是一个

背景情况:

导向基因 (DIR) 基因家族对于植物适应环境变化至关重要.
对玉米DIR基因 (ZmDIRs) 的研究是有限的,特别是关于非生物应激反应的研究.
ZmDIR5被确定为玉米应激耐受性的潜在关键因素.

研究的目的:

研究ZmDIR5在玉米对非生物应激反应中的作用.
描述ZmDIR5在调节植物生长和耐压力机制中的功能.
阐明ZmDIR5参与压力反应途径的基础分子机制.

主要方法:

在玉米中识别和描述ZmDIR5.
在浸水,盐和干旱压力条件下分析ZmDIR5表达.
ZmDIR5乙基甲硫酸盐 (EMS) 突变系的生成和表型分析.
生物化学试验测量反应性氧物种,甲,林,甲酸和抗氧化酶活性.
对离子运输,激素水平 (酸,泽) 和基因表达的分析.
对烯胺生物合成途径的研究.

主要成果:

ZmDIR5的表达因水浸,盐和干旱压力而上调.
ZmDIR5蛋白位于内质网膜中.
与野生型植物相比,ZmDIR5-EMS突变体表现出减少的生长和受损的应激耐受性.
突变系显示氧化应激增加 (马隆迪化物,活性氧物种) 和抗氧化能力降低.
在突变者中观察到有损的离子运输,降低了酸和氨酸的合成,以及抑制了烯胺生物合成.
ZmDIR5作为玉米耐水,盐和干旱压力的积极调节者.

结论:

ZmDIR5在提高玉米对多种非生物压力的耐受性方面发挥着重要作用.
ZmDIR5的功能包括调节氧化应激,离子稳态,激素合成和烯胺生物合成.
ZmDIR5是提高玉米抗压能力的有价值目标.

关键词:

DIRs 是一个DIR.在 ZmDIR5 里面,你会看到 ZmDIR5.干旱造成的压力是干旱.盐的耐受性盐的耐受性浸水耐受性的水浸水.

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