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尼尔-1 通过对NF-κB通路的调节来抑制脂多糖激活的巨细胞转化为M1表型

Yue Chen¹, Qiang Wang², Mengyue Li¹

¹School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Research Center of Dental Materials and Oral Tissue Regeneration & Shandong Provincial Clinical Research Center for Oral Diseases, No.44-1 Wenhua Road West, Jinan, 250012, Shandong, China.

Journal of molecular histology

|March 17, 2025

概括

类似Nell-1的分子-1 (Nell-1) 通过抑制M1巨细胞极化和促进M2标记物来减少炎症. 这种分子调节NF-κB通路,显示出治疗炎症疾病和组织再生的潜力.

科学领域:

生物化学生物化学
免疫学免疫学免疫学
分子生物学分子生物学

背景情况:

奈尔样分子-1 (Nell-1) 是一种具有多种功能的骨质诱导因子,包括抗炎性质.
尼尔-1的抗炎机制,特别是巨细胞中的抗炎机制以及它在巨细胞两极分化中的作用,需要进一步阐明.

研究的目的:

为了研究Nell-1在人类髓状细胞系 (THP-1) 衍生的巨细胞中的抗炎作用.
探索Nell-1在调节巨细胞极化及其潜在分子机制中的作用.

主要方法:

THP-1细胞分化为巨细胞,并用Nell-1和脂多糖 (LPS) 进行治疗.
分析了M1和M2巨细胞标记物 (CD86,TNF-α,IL-6,IL-1β,VEGF,Arg-1,CD206) 的情况.
评估了Nell-1对LPS诱导的核因子-kappa B (NF-κB) 转位的影响.

主要成果:

尼尔-1显著降低了M1标记物 (CD86) 和促炎细胞因子 (TNF-α,IL-6,IL-1β).
尼尔-1通过上调M2标记物 (VEGF,Arg-1,CD206) 来逆转LPS诱导的M1极化.
尼尔-1抑制了LPS诱导的NF-κB转位,表明NF-κB通路调节.

结论:

尼尔-1通过调节从M1到M2表型的极化,在巨细胞中表现出显著的抗炎作用.
尼尔-1的抗炎作用是通过调节NF-κB信号通路来调节的.
尼尔-1具有作为治疗炎症疾病和促进组织再生的治疗剂的潜力.

关键词:

抗炎药物抗炎药物抗炎药物巨细胞的两极分化通过NF-κB路径.尼尔-1 尼尔-1 是一个在 THP-1 巨细胞中.

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