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动态蛋白质组和乙烯基组分析揭示了甘中糖积累的关键调节者

Miao Wang^1,2, Ao-Mei Li¹, Zhong-Liang Chen¹

¹Guangxi Key Laboratory of Sugarcane Genetic Improvement, Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture and Rural Affairs, Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, 530007, Guangxi, China.

Plant cell reports

|March 17, 2025

概括

lysine 乙化和蛋白质水平调节了甘糖的积累. 研究人员确定了73种关键蛋白质,为分子育种提供了新的点,以提高糖含量.

科学领域:

植物生物学植物生物学
生物化学生物化学
分子育种是一种分子育种.

背景情况:

氨酸乙化调节植物生物过程,但其在甘糖积累中的作用尚不清楚.
甘中糖的含量对农业和工业至关重要.

研究的目的:

调查蛋白质丰富度和氨酸乙化在甘糖累积中的作用.
确定潜在的分子标,以提高甘中的糖水平.

主要方法:

甘叶的全面定量蛋白质组和乙化蛋白质组分析.
在不同发育阶段,高糖和低糖基因型之间的蛋白质丰度和乙化模式的比较.

主要成果:

鉴定了2363种不同丰富的蛋白质 (DAP),其中165种与糖代谢有关.
鉴定了1397个具有2377个乙化位点的差异性乙化蛋白 (DAcPs),其中许多参与糖代谢.
发现了73种与糖代谢相关的重叠蛋白质 (DAP和DAcP),表明它们具有双重调节作用.

结论:

蛋白质丰富度和氨酸乙化都对甘的糖积累至关重要.
73种已识别的蛋白质代表了分子育种的有希望的目标,以提高甘中糖含量.
希斯乙化和代谢也可能影响糖的积累.

关键词:

乙化乙化是一种有差异的丰富蛋白质.不同地乙化蛋白质的不同.蛋白质组学是指蛋白质组学.糖的积累糖的积累甘是一种糖.

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Oligosaccharide Assembly

Oligosaccharide Assembly

Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
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