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EIF4A3-诱导circ_0029941 通过通过miR-224-5p/NFAT5轴增强自细胞激活促进星细胞激活

Chonghua Jiang¹, Hui Liu², Jun Peng¹

¹Department of Neurosurgery, Haikou Affiliated Hospital of Central South University Xiangya School of Medicine Haidian Island, No.43, People's Avenue, Hainan Province, Haikou City, 570203, People's Republic of China.

Molecular neurobiology

|March 17, 2025

概括

循环RNAcirc_0029941通过增加天体细胞激活和自促进急性缺血性中风 (AIS). 抑制circ_0029941可能为AIS治疗提供治疗策略.

科学领域:

分子生物学分子生物学
神经科学是一个神经科学.
遗传学是一个遗传学.

背景情况:

异常循环RNA (circRNA) 表达与人类疾病有关,包括急性缺血性中风 (AIS).
在AIS进展中circ_0029941的特定作用和调控机制尚未完全理解.

研究的目的:

研究circ_0029941在急性缺血性中风 (AIS) 进展中的功能和机制.
在AIS中探索涉及circ_0029941,miR-224-5p和NFAT5的调节途径.

主要方法:

已建立的AIS小鼠模型使用短暂的中脑动脉封闭 (tMCAO) 和体外星球细胞模型与氧气-葡萄糖剥夺/氧化 (OGD/R).
使用定量实时PCR对circ_0029941,EIF4A3,miR-224-5p和NFAT5的量化表达水平进行了量化实时PCR.
通过组织学染色,西部斑点,RIP,RNA下拉和双露西法酶记者测定评估了心脏病发作大小,星球细胞激活,自标记物和蛋白质表达.

主要成果:

在AIS患者,tMCAO模型和OGD/R诱导的星球细胞中,circ_0029941的表达显著升高.
在AIS模型中, Knockdown of circ_0029941减少了脑梗塞,抑制了天体细胞激活,并抑制了ATG5介导的自.
EIF4A3促进了circ_0029941的表达,它作为miR-224-5p的海绵来调节NFAT5,从而促进了天体细胞激活和自.

结论:

在AIS中,EIF4A3诱导的circ_0029941通过miR-224-5p/NFAT5轴促进星体细胞激活和自.
Circ_0029941代表了缓解急性缺血性中风中脑损伤的潜在治疗目标.

关键词:

急性缺血性中风是一次急性缺血性中风.星球细胞是星球细胞.自自是一种自的过程.圆周_002994141 在线阅读欧洲投资基金4A3A3

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