在患有巨细胞动脉炎的患者中,Granzyme B产生调节性B细胞
Marian Stöcker1, Uta Kiltz2, Jürgen Braun2
1Department of Pneumology and Infectious Diseases, Thoraxzentrum Ruhrgebiet, Herne; Ruhr-University Bochum; and Department of Nephrology, University Hospital Essen, University of Duisburg-Essen, Essen, Germany. marian9207@arcor.de.
Clinical and experimental rheumatology
|March 17, 2025
概括
在巨细胞动脉炎 (GCA) 患者中,产生大酶B (GrB) 的调节性B细胞减少,这表明B细胞抑制能力受损. 与健康对照组相比,产生调节性B细胞的介质素-10 (IL-10) 在GCA患者中没有差异.
科学领域:
- 类风湿病学 类风湿病学
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
背景情况:
- 巨细胞动脉炎 (GCA) 是一种严重的炎症性风湿性疾病.
- T细胞驱动GCA的发病,而调控性B细胞 (Breg) 具有抗炎性质.
- 在GCA中Breg的特定功能仍然不充分理解.
研究的目的:
- 为了研究GCA患者的抗炎B细胞区.
- 为了评估GCA中的调控性B细胞产生的花粉酶B (GrB) 和互白素-10 (IL-10) 的产生.
主要方法:
- 从GCA患者 (n=47) 和健康对照 (HC) (n=49) 中分离了周围血液单核细胞 (PBMC).
- 调控性B细胞 (Breg) 在体外被刺激以评估GrB和IL-10的产生.
- 还分析了T细胞细胞因子的产生,包括IFNγ.
主要成果:
- 与HC相比,在GCA患者中,产生GrB的Breg的比例显著低于GCA患者,无论疾病活性如何.
- 在GCA患者和HC患者之间没有观察到IL-10产生Breg的显著差异.
- 患有活跃疾病的GCA患者的CD4+T细胞产生的IFNγ比HC少.
结论:
- 在GCA患者中,调节性B细胞发生变化,GrB产生Breg.的持续减少.
- 在产生IL-10的Breg中缺乏差异表明在B细胞中介抑制中存在潜在的缺陷.
- 这些Breg的变化可能会影响GCA病变的T细胞区.
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