转录异质性塑造了酵母体的应激适应反应
Mariona Nadal-Ribelles1,2, Guillaume Lieb3, Carme Solé4,5
1Department of Medicine and Life Sciences, Universitat Pompeu Fabra, Barcelona, 08003, Spain. mariona.nadal@irbbarcelona.org.
Nature communications
|March 18, 2025
概括
细胞对压力的反应显示出令人惊的基因表达变异性. 这项研究揭示了酵母透适应中的这种异质性如何产生独特的适应性策略,以提高细胞存活率.
科学领域:
- 细胞和分子生物学 细胞和分子生物学
- 遗传学和基因组学 遗传学和基因组学
- 系统生物学 系统生物学
背景情况:
- 细胞通过改变基因表达激活信号通路,以生存压力.
- 即使在类似的压力条件下,在基因相同的细胞中也存在显著的基因表达变异.
- 这种细胞变化的分子基础和适应意义仍然不完全理解.
研究的目的:
- 用单细胞RNA测序在酵母中透适应过程中纵向研究转录动力学.
- 了解驱动基因表达异质性的机制,以应对透性压力.
- 确定影响转录变异性的遗传因素及其对细胞适应性的影响.
主要方法:
- 纵向单细胞RNA测序 (scRNA-seq) 来追踪转录变化.
- 对基因表达模式和组合表达程序的分析.
- 使用RNA条形编码对300多种删除突变的高通量选.
主要成果:
- 度适应涉及度响应程序的高度异质表达,形成不同的细胞程序.
- 应激时的全球转录组抑制促进了这些适应性程序的诱导.
- 具有基底表达的细胞的 osmoresponsive 程序表现出增加的抗压力和反应能力.
- 鉴定了影响转录组异质性的遗传因素和应激响应子群体的调节者.
- 转录异质性与改善细胞适应性有关.
结论:
- 转录异质性是酵母透适应的一个关键特征,产生各种适应性策略.
- 全球转录组抑制在有利于特定的自适应程序诱导方面发挥着作用.
- 了解异质性的遗传基础,可以了解细胞的健康状况和应激反应.
- 这项研究提供了酵母透适应中的转录表型的调控图.
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