相关实验视频

Updated: May 21, 2025

Isolation of Human Endometrial Stromal Cells for In Vitro Decidualization

Isolation of Human Endometrial Stromal Cells for In Vitro Decidualization

Published on: September 1, 2018

精液等离子体外基因组衍生的miR-26-5p可以通过PTEN / PI3K / AKT信号通路调节状巨细胞极化

Yan Zhang¹, Xiaolin Chen², Jie Li¹

¹Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei, China.

Scientific reports

|March 18, 2025

概括

精液等离子体 (SP) 通过精液外体 (SE) 和miR-26-5p促进了状巨细胞的M2两极分化. 这增强了免疫耐受性,改善了胚胎植入和发育,为怀孕结果提供了新的治疗途径.

科学领域:

生殖免疫学生殖免疫学
母胎免疫相互作用母胎免疫相互作用
细胞和分子生物学是细胞和分子生物学.

背景情况:

精液等离子体 (SP) 免疫调节对于胚胎植入至关重要.
具有M2两极分化的果性巨细胞 (dMΦs) 支持怀孕的维持.
SP对dMΦ偏振的影响尚不清楚.

研究的目的:

研究SP在dMΦ极化中的作用和机制.
探索SP对母胎免疫微环境的影响.
评估SP在改善妊娠结果方面的潜力.

主要方法:

基因芯片测序用于识别精子外体 (SE) 中的关键分子.
在体外实验中评估dMΦ极化.
在自发流产的小鼠模型中的体内研究.

主要成果:

SP显著促进了dMΦs的M2极化.
在SEs中高度表达的miR-26-5p被确定为一个关键的媒介.
在小鼠模型中,SEs补充剂减少了胚胎再吸收.

结论:

在SP中,SE衍生的miR-26-5p通过PTEN/PI3K/AKT通路促进dMΦ M2极化.
这创造了一个对胚胎发育至关重要的免疫耐受性环境.
基于SP的治疗有望改善妊娠结果.

关键词:

胚胎的植入和发育过程巨细胞的极性性精子外生体是精子外生体.精液等离子体中的精液.在 miR-26-5p 中使用.

更多相关视频

Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae

Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae

Published on: April 30, 2018

Isolation of Mouse Endometrial Epithelial and Stromal Cells for In Vitro Decidualization

Isolation of Mouse Endometrial Epithelial and Stromal Cells for In Vitro Decidualization

Published on: March 2, 2017

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...