通过瘤内皮进行纳米粒子运输的途径
Jamie L Y Wu1,2, Qin Ji1,2, Colin Blackadar1,2
1Institute of Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada.
Nature nanotechnology
|March 18, 2025
概括
纳米颗粒通过巨细胞细胞结合 (macropinocytosis) 活跃地被运送到瘤中,这一过程涉及细胞膜卷捕获和内化纳米颗粒. 这种活跃的传输机制增强了纳米粒子向瘤的传递.
科学领域:
- 生物医学工程 生物医学工程
- 纳米技术纳米技术
- 癌症生物学 癌症生物学
背景情况:
- 增强透性和保留 (EPR) 效应是纳米粒子瘤输送的广泛接受的模型.
- 然而,主动运输和保留原理为纳米粒子在瘤中的积累提供了另一种解释.
- 纳米颗粒穿越瘤内皮细胞的确切机制尚不清楚.
研究的目的:
- 阐明纳米颗粒用于穿越瘤内皮细胞的主要途径.
- 为了研究膜和内细胞分裂在穿过瘤内皮的纳米粒子运输中的作用.
- 了解纳米粒子大小如何影响不同运输途径的贡献.
主要方法:
- 利用先进的显微镜技术观察纳米粒子-内皮细胞相互作用 in vivo 和 in vitro.
- 研究了纳米粒子内部化中巨型皮诺细胞体和受体介导内细胞体的作用.
- 分析了纳米粒子大小对不同运输途径效率的影响.
主要成果:
- 证明纳米颗粒主要通过非受体基的巨细胞结核细胞穿越瘤内皮细胞.
- 识别了瘤内皮细胞膜的,作为捕获和内化纳米粒子的关键结构.
- 观察到纳米颗粒大小显著影响了宏皮诺细胞体与克拉林介导的内细胞体的贡献.
- 与健康的内皮相比,在瘤内皮中发现了更高的膜缩密度.
结论:
- 巨型皮诺细胞体是穿越瘤内皮细胞的纳米粒子运输的主要机制.
- 瘤内皮细胞膜纹在纳米粒子吸收和随后释放到瘤间隙中发挥着关键作用.
- 了解这些传输机制对于优化基于纳米粒子的癌症疗法和药物输送系统至关重要.
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