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来自中国Curvularia lunata的一种新型真菌病毒的分子表征

Bin Ke¹, Xiaohan Mo², Mengting Wu¹

¹State Key Laboratory for Quality and Safety of Agro-products, Key Laboratory of Biotechnology in Plant Protection of MARA, Zhejiang Key Laboratory of Green Plant Protection, Institute of Plant Virology, Ningbo University, Ningbo, 315211, China.

Archives of virology

|March 18, 2025

概括

一种新型的真菌病毒,Curvularia lunata RNA病毒1 (ClRV1),在中国被发现. 它的基因组分析表明,它可能需要一种新的病毒序列.

科学领域:

菌类学菌类学是指菌类学.
病毒学病毒学
基因组学就是基因组学.

背景情况:

菌类病毒是感染真菌的病毒.
曲流 (Curvularia lunata) 是一种真菌物种.
了解真菌病毒的多样性对于真菌生物学至关重要.

研究的目的:

在Curvularia lunata中识别和描述一种新型的真菌病毒.
确定新发现病毒的完整基因组序列.
为了研究新型病毒与已知的真菌病毒的遗传关系.

主要方法:

高通量测序用于最初的病毒发现.
反转录聚合酶连锁反应 (RT-PCR) 用于确认和分析.
进行了BLASTn和BLASTp搜索以进行序列相似性比较.
用病毒RNA依赖RNA聚合酶 (RdRp) 序列进行了基因组学分析.

主要成果:

一种新的真菌病毒,Curvularia lunata RNA病毒1 (ClRV1) 被确定.
ClRV1的完整基因组是3,742个核酸,有两个开放的读取框架 (ORF).
ClRV1与Erysiphe necator相关的状病毒4 (EnUlV4) 具有显著的序列相似性,特别是在其HP和RdRp蛋白中.
遗传学分析将ClRV1与其他两病毒,真菌病毒和乌姆布拉米病毒联系在一起,这表明在Kitrinoviricota中存在潜在的新病毒序列.

结论:

这项研究报告了第一个感染Curvularia lunata的ClRV1的完整基因组序列.
基因组和遗传学数据支持这些相关的真菌病毒建立一个新秩序.
这一发现扩大了已知的真菌病毒的多样性及其进化关系.

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