没有母体血细胞的DNA核细胞足迹可以揭示怀孕期间和子宫前期间基因表达特征的变化
Kun Li1, Zhiwei Guo1, Fenxia Li2
1Institute of Antibody Engineering, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, Guangdong, 510515, P. R. China.
BMC pregnancy and childbirth
|March 18, 2025
概括
没有母体血细胞的DNA核细胞足迹提供了一种非侵入性方法来检测怀孕期间和妊娠前的基因表达变化,作为并发症的潜在生物标志物.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 生物标志物发现发现
背景情况:
- 不同的基因表达对于理解怀孕及其并发症至关重要.
- 目前分析胎儿和胎盘基因表达的方法是侵入性的.
- 无等离子细胞DNA (cfDNA) 核细胞足迹反映了来自母亲血液和胎盘的基因表达.
研究的目的:
- 验证使用血cfDNA核细胞足迹来检测差异表达基因 (DDG).
- 评估cfDNA核细胞足迹作为怀孕和子宫前的非侵入性生物标志物的潜力.
主要方法:
- 从非怀孕的,健康的孕妇和前性妇女的血cfDNA深度测序.
- 在基因促销器区域中提取和规范化读数.
- 基因深度的统计比较以确定DDG和功能丰富分析.
主要成果:
- 在三个组的血cfDNA中观察到明显的核细胞足迹.
- 孕妇和非孕妇之间的629个DDG主要涉及免疫调节.
- 1978年,健康孕妇和孕妇产前期之间的DDG显示出免疫调节,细胞循环和感官感知方面的差异.
结论:
- 血cfDNA核细胞足迹深度与怀孕期间和子宫前时的基因表达变化相关.
- 这种方法显示了作为一种非侵入性生物标志物,用于怀孕状态和诸如子宫前等并发症.
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