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植物生成-代谢双向单细胞基因组学解剖和采矿生态系统功能由FISH-scRACS-seqq

Xiaoyan Jing^1,2,3,4, Yanhai Gong^1,2,3,4, Zhidian Diao^1,2,3,4

¹Single-Cell Center, CAS Key Laboratory of Biofuels, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266000, China.

Innovation (Cambridge (Mass.))

|March 18, 2025

概括

一种新的单细胞基因组学方法,FISH-scRACS-seq,可以直接从环境样本中识别微生物功能和基因组. 这种方法在γ-蛋白质细菌中发现了新的环素降解酶,进步了我们对微生物新陈代谢的理解.

科学领域:

微生物生态学微生物生态学
基因组学就是基因组学.
代谢学代谢学代谢学

背景情况:

微生物组范围的关联研究 (MWAS) 识别了生态系统特征的微生物标记物,但功能机制和个体基因组仍然难以确定.
验证*in situ*代谢活动并将其与特定的基因组联系起来,是微生物研究中的一个重大障碍.

研究的目的:

开发和验证一种新的单细胞基因组学方法,用于剖析微生物功能并将其与特定的基因组联系起来.
为了确定在污染的海水中负责环素降解的特定酶和生物.

主要方法:

引入光在位混合导向的单细胞拉曼激活分类和测序 (FISH-scRACS-seq),一种细胞生成-代谢双向单细胞基因组学方法.
FISH-scRACS-seq能够直接定位目标种群,通过拉曼光谱对*in situ*代谢功能进行分析,对具有特定代谢活动的细胞进行分类,并生成高覆盖率的单细胞基因组.
将FISH-scRACS-seq应用于被环素污染的海水中,以识别和测序活性降解环素的γ-蛋白细菌.

主要成果:

FISH-scRACS-seq成功识别和分类了积极降解环素的γ-蛋白质细菌细胞 *in situ*.
一个单个*Pseudoalteromonas fuliginea*细胞的全基因组测序揭示了一种新的三组分细胞染色体P450系统.
这种新发现的系统被证实在体外*将环素转化为环醇,代表了以前未知的环素降解途径和生物群.

结论:

FISH-scRACS-seq是一种强大且普遍适用的方法,用于在单细胞分辨率下剖析微生物功能.
该研究揭示了具有生态相关性的新型酶,途径和基因组,特别是确定了新的环素降解剂.
这种方法显著提高了挖掘微生物群功能的能力,并了解生态重要生物体的细胞活动.

关键词:

鱼类鱼类是一种鱼类.光在现场混合化中的光.雷克斯 (RACS) 是一个拉曼激活细胞分类拉曼激活细胞分类单细胞拉曼显微光谱学我们的微生物群.污染物的降解降解污染物.

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