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Updated: May 21, 2025

Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR
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通过rRNA基因安普利康序列测序对微生物群的跨域绝对量化进行合成DNA入标准

Dieter M Tourlousse1, Yuji Sekiguchi1

  • 1Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.

ISME communications
|March 18, 2025
PubMed
概括

新的rDNA模仿物在微生物组研究中实现了精确,绝对的量化. 这些合成标准准确地测量了真菌和细菌负载,克服了差异微生物分析的相对丰度限制.

关键词:
绝对量化的绝对量化.跨领域的微生物组分析.微生物组是一个微生物组.rRNA基因安普利康序列的测序合成的spike-in标准的使用.

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