探索Paenibacillus terrae B6a作为Fusarium proliferatum的一个可持续的生物控制剂
Enriquay Smith1, Augustine Innalegwu Daniel1,2, Chelsey Smith3
1Plant Omics Laboratory, Department of Biotechnology, Faculty of Natural Sciences, University of the Western Cape, Bellville, South Africa.
Frontiers in microbiology
|March 18, 2025
概括
Paenibacillus terrae B6a有效控制植物病原体Fusarium proliferatum,通过抑制其生长和减少玉米中的压力. 这项研究强调了B6a作为可持续农业的有希望的生物杀菌剂.
科学领域:
- 农业微生物学 农业微生物学
- 植物病理学 植物病理学
- 生物控制剂 生物控制剂
背景情况:
- 化学杀菌剂带来环境和健康风险,需要可持续的替代品.
- 使用对抗性微生物进行的生物控制为管理植物疾病提供了一种环保的方法.
- 虫 (Fusarium proliferatum) 是一种影响作物产量的重要植物病原体.
研究的目的:
- 评估Paenibacillus terrae B6a作为一种生物控制剂,用于对抗Fusarium proliferatum.
- 评估B6a的体外对抗活性和植物内疗效.
- 研究B6a对真菌形态,酶含量和玉米植物应激反应的影响.
主要方法:
- 试验室双培养和代谢物测试以确定对F. proliferatum的对抗活性.
- 高分辨率扫描电子显微镜 (HR-SEM) 观察真菌形态变化.
- 在植物用B6a对玉米种子进行生物原始化,随后进行F. proliferatum感染和根长度,基,细胞外多糖,内分β-1,4-葡萄糖酶和活性氧物种 (ROS) 的分析.
主要成果:
- 在实验室中,B6a显著抑制了F. proliferatum (70.15-71.64%).
- B6a改变了F. proliferatum的形态,增加了基含量,并减少了细胞外多糖和内-β-1,4-葡萄糖酶活性.
- 在植物中,B6a显著增加了玉米根长度 (44.99%),并减少了与受感染对照相比,ROS诱导的氧化损伤.
结论:
- 帕尼巴西勒斯地B6a表现出强大的生物控制能力,可以对抗Fusarium proliferatum.
- B6a有效地减轻了病原体引起的压力,并促进了玉米的植物生长.
- P. terrae B6a是开发生物杀菌剂的可行候选者,用于可持续的作物保护.
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