(+) -和 (-) -奥兰丁的酶选择生物合成
Shinji Kishimoto1, Tsubasa Tamura1, Takumi Okamoto1
1Department of Pharmaceutical Sciences, University of Shizuoka, Shizuoka 422-8526, Japan.
Journal of the American Chemical Society
|March 18, 2025
概括
菌株通过不同的生物合成途径产生天然产物. 这项研究揭示了菌中独特的含氨基酸生物合成和化物天然产品的产生.
科学领域:
- * 菌类学和自然产品化学
- * 生物化学和分子生物学
背景情况:
- 生物活性天然产品 (NP) 通常在生物合成过程中受到立体化学控制.
- 由于酶的立体特异性,在真菌代谢物中很少发现异构体 (镜像异构体).
- * 奥兰丁是一种含有亚基的真菌NP,其生物合成以前未被描述.
研究的目的:
- * 为了研究不同Aspergillus lentulus菌株产生的奥兰反体的生物合成.
- * 确定产生 (-) - 和 (+) - 氨酸异构体的基因和酶.
- * 阐明菌中含氨基酸生物合成的机制.
主要方法:
- *转录组分析以确定相关的基因.
- *基因删除和异质表达实验以确认基因功能.
- * 生物化学测定以鉴定酶活性.
主要成果:
- * 确定了两个不同的非核糖体合成酶 (NRPS),分别负责合成 (-) - 和 (+) - 氨酸的Nita和NitC.
- * 发现NitC具有表皮化域,解释了酶体对的产生.
- * 具有特征的NitB,一种类似于酸合成酶,可从l- glutamine中催化含有酸的氨基酸l-γ- cyanohomoalanine.
结论:
- 通过不同的NRPS酶 (NitA和NitC),不同的Aspergillus lentulus菌株产生奥兰的反体.
- * 在NitC中存在一个表皮化域是产生基对的关键.
- * 发现了一种涉及NitB的含氨基酸生物合成的新途径.
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