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对从加利福尼亚海中分离出来的Campylobacter jejuni菌株的毒性和传染性评估
Jingrang Lu1, David Linz2, Ian Struewing1
1U.S. EPA Office of Research and Development, Cincinnati, Ohio, USA.
Microbiology spectrum
|March 18, 2025
概括
野生鸟类Campylobacter jejuni (C. jejuni) 菌株,如来自加利福尼亚海的63A分离物,显示出高毒性潜力. 这种C. jejuni分离物具有关键的毒性基因,并具有动物感染潜力,对人类健康构成风险.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 公共卫生 公共卫生
背景情况:
- 野生鸟类是Campylobacter jejuni (C. jejuni) 的已知的储存库,这是导致食物传播疾病的首要原因.
- 有限的数据存在于野生鸟类相关的地表水中的C. jejuni菌株的毒性和人类健康风险.
- 了解这些孤立的鸟类中毒性的遗传基础对于评估公共卫生威胁至关重要.
研究的目的:
- 调查C.病毒的毒性和动物感染潜力. 杰尼菌株63A从加利福尼亚海排泄物中分离出来.
- 将63A菌株的遗传构成与致病性C. jejuni菌株进行比较,特别是与吉林巴雷综合征 (GBS) 相关的菌株.
- 确定63A菌株的传染剂量,并评估其感染人类的潜力.
主要方法:
- 全基因组测序和分析C. 63A. jejuni 63A. jejuni 63A. jejuni 63A. jejuni 63A. jejuni 63A.
- 多位数序列类型 (MLST) 来确定序列类型 (ST2654).
- 对病毒性相关基因 (LOS,CPS,cdtABC) 与临床分离物进行比较的基因组分析.
- 遗传学分析以评估宿主范围.
- 在小中进行剂量反应实验,以估计中位传染剂量 (MID).
主要成果:
- 在C.I.C.中, 63A (ST2654) 呈现出高DNA序列相似性 (97%-99%) 与致病性C. jejuni菌株,包括来自GBS患者的菌株.
- 63A菌株具有完整的脂聚糖体 (LOS),囊多糖体 (CPS) 基因集群和细胞张毒素 (CDT) 操作子 (cdtABC),与GBS相关的临床分离物保持着.
- 在63A中发现的独特的LOS基因将其置于1组LOS位点类型中,与许多其他野生鸟类隔离物不同.
- 遗传学分析表明63A与包括沙丘起重机在内的多种鸟类宿主有密切的关系,这表明它具有广泛的储存潜力.
- 剂量反应分析确定中位传染剂量为7.16 × 10 7 CFU,证实了动物感染潜力.
结论:
- 在C.I.C. 来自加利福尼亚海的63A分离体显示出显著的毒性潜力,这是由于关键毒性因子的存在和保存.
- 菌株63A与人类致病菌株具有关键的遗传相似性,特别是那些引起吉兰-巴雷综合征的菌株,突出显示了直接的公共卫生问题.
- 已识别的动物感染潜力和C.C.的广泛宿主协会. 63A强调了监测野生鸟类种群对可传播到人类的病原体的重要性.
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