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尿液外体体中的细胞特异蛋白表达作为后COVID状态中脏损伤的标记物
Seshagiri Rao Nandula1, Beda Brichacek1, Sabyasachi Sen1
1Department of Medicine and Biochemistry, School of Medicine and Health Sciences, The George Washington University, Washington, DC, USA.
Metabolic syndrome and related disorders
|March 18, 2025
概括
COVID-19 感染可能会导致持续的损伤,急性疾病后很长一段时间内,尿液中的 podocyte 蛋白质如 Podocalyxin 和 Nephrin 的升高表明了这一点. 这表明COVID-19后患慢性病的潜在风险.
科学领域:
- 腎臟病學 (nephrology) 是一種醫學專業.
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
背景情况:
- 严重急性呼吸系统综合征 冠状病毒-2 (SARS-CoV-2) 导致COVID-19,可能导致内皮细胞功能障碍,心脏代谢疾病和 podocytopathy.
- 了解COVID-19后的长期损伤至关重要,特别是在细胞损伤及其与高血糖症的关联方面.
研究的目的:
- 为了调查高血糖是否会导致SARS-CoV-2感染.
- 为了确定COVID-19是否会增加长期持续性损伤的风险,特别是 podocytopathy.
- 在急性COVID-19感染后的不同时间点在尿液外体中评估 podocyte 特定蛋白质水平.
主要方法:
- 从乔治华盛顿大学的COVID存储库收集了SARS-CoV-2感染后患者的血液和尿液样本.
- 隔离的外周血液单核细胞和尿液外体.
- 在感染后的10天,6个月和12个月,测量了 podocyte 特定蛋白质 Podocalyxin (PODXL) 和 Nephrin (NEPH) 的尿外体水平.
主要成果:
- 在COVID-19后的10周,6个月和12个月观察到尿液中异位体波多卡辛水平显著升高.
- 在COVID-19后10周和12个月观察到尿液外体内氨酸水平升高.
- 尽管皮囊细胞蛋白质含量高,但尿白蛋白:肌素比率 (UACR) 与对照组相比没有显著差异.
结论:
- 在COVID-19后12个月内,尿中高水平的尿细胞特异性蛋白质表明正在进行的细胞损伤.
- 这些发现表明,在COVID-19感染后,可能存在长期患慢性病的风险.
- 需要进一步的研究来澄清高血糖在SARS-CoV-2感染中的作用及其对结局的影响.
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