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精子tRNA衍生碎片的表达可能与禁欲相关的精子质量改善有关

Xi-Ren Ji^1,2, Rui-Jun Wang¹, Zeng-Hui Huang²

¹NHC Key Laboratory of Human Stem Cell and Reproductive Engineering, School of Basic Medical Sciences, Central South University, Changsha 41008, China.

Asian journal of andrology

|March 18, 2025

概括

短暂的禁欲期显著提高了精子的运动性,并减少了DNA损伤. 转移RNA衍生的片段 (tRFs) 起着关键作用,为男性生育提供潜在的生物标志物,并优化辅助生殖技术 (ART).

科学领域:

生殖生物学生殖生物学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

短暂的射精禁欲期可能会改善精子参数,但潜在的分子机制尚未完全理解.
在辅助生殖技术 (ART) 中,优化精液质量至关重要.

研究的目的:

调查缩短禁欲期 (1-3小时) 对精液质量的影响.
为了探索分子机制,特别是转移RNA衍生碎片 (tRFs),参与精子参数改进.

主要方法:

分析不同禁欲期后 (3-7天 vs 1-3小时) 具有正常和低精子参数的男性的精液样本.
评估生理和生化精子参数的评估.
深度RNA测序以分析与禁欲期相关的tRF动态.
生物信息分析以确定差异表达的tRF和丰富的信号通路.

主要成果:

1-3小时的禁欲期显著改善了精子的运动性,并降低了DNA碎片化指数.
鉴定了245个差异表达的tRF,其中基因激活蛋白激酶 (MAPK) 信号通路是最丰富的.
特定的tRF-Lys-TTT及其标MAP2K2显示出显著的变化,这表明它在增强精子功能方面发挥了作用.

结论:

减少射精禁欲期可以提高精子质量,特别是运动性和DNA完整性.
tRFs,特别是tRF-Lys-TTT,与改善精子功能的分子机制有关.
tRFs可以作为男性生育能力的新生物标志物,并为优化ART协议提供目标.

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