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相关概念视频

Synthetic Biology02:55

Synthetic Biology

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Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
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Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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相关实验视频

Updated: May 21, 2025

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
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Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

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在常用的合成生物学等离子体中防止多重构成.

Elizabeth Vaisbourd1, Anat Bren1, Uri Alon1

  • 1Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel 76100.

ACS synthetic biology
|March 18, 2025
PubMed
概括

等离子体多重体,等离子体的双重重复,在Escherichia coli MG1655中形成,但在JM109.9中不典型. 一个 ΔrecA 淘汰压力防止了多元体的形成,提高了合成电路的可靠性.

科学领域:

  • 分子生物学分子生物学
  • 合成生物学 合成生物学
  • 微生物学 微生物学

背景情况:

  • 等离子体是研究和生物技术中的重要工具.
  • 维护等离子体的完整性,特别是防止多重体的形成,对于优化合成电路至关重要.
  • 由于二元分辨率失败而产生的等离子体多元体,可以影响电路功能,特别是在DNA编辑酶方面.

研究的目的:

  • 为了调查等离子体多元体在常见的实验室菌株中的发生.
  • 识别影响多重体形成的因素.
  • 开发策略,以减轻基于等离子体的系统中的多聚合物生产.

主要方法:

  • 在克隆 (JM109) 和野生型 (MG1655) Escherichia coli 菌株中调查了四个常见的等离子体骨架.
  • 评估了与等离子体拷贝数和培养通过有关的多重分子形成.
  • 创建并测试了一种MG1655 ΔrecA单位淘汰菌株,以检测其防止多分子生产的能力.

主要成果:

  • 只有在MG1655菌株中观察到有意义的等离子体多元体.
  • 在MG1655.5中,多重分子分数随着更高的等离子体拷贝数和延长的培养经过而增加.
  • 将多元体转化为JM109导致了缺乏单片质粒的菌株.
关键词:
其他 concatemers 的情况.长时间阅读序列排序.一个多元化的多元化器.纳米孔测序的测序塑料体中的塑料.再组合的复合方式.

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Last Updated: May 21, 2025

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  • MG1655 ΔrecA淘汰菌株有效地防止了多重体的形成.

    • 结论:

    • 在大肠杆菌中,等离子体多元化取决于菌株,特别是在野生型MG1655.5中发生.
    • 诸如等离子体拷贝数和传递等因素影响了多分子积累.
    • 基因改造,例如创建一个 ΔrecA 淘汰,可以消除多重体生产,提高基于等离子体的合成电路可靠性.